摘要
采用了RAPD、RGA和SRAP分子标记技术对22个番茄品种进行了分子鉴别的研究。通过引物筛选,选用了7个RAPD随机引物,扩增后共产生了17个多态性位点;2对RGA引物,产生了12个多态性位点;1对SRAP引物,产生了8个多态性位点。分析单引物的品种鉴别能力发现,RGA和SRAP标记具有较高的鉴别效率。为了鉴别22个番茄品种,分析了双引物组合和三引物组合的鉴别能力,双引物组合不能完全区分22个番茄品种,利用引物BI26和PK1+PK2再加上S91或S92中的任一个就可将22个番茄品种区分开来,并获得各品种独特的核酸指纹。另外,通过聚类分析发现,现代番茄品种之间存在着较为复杂的亲缘关系,用有限的标记位点很难取得理想的聚类结果。
Three PCR-based systems, including RAPD, RGA and SRAP were employed to differentiate 22 tomato varieties. In this study, laborious work was conducted in primer selection, 17 polymorphic loci were produced by 7 RAPD primers, 12 polymorphic loci by 2 RGA primer pairs, and 8 polymorphic loci by one SRAP primer pairs. The informative loci by each primer or primer pair were used to identify 22 tomato varieties, respectively. It showed that the RGA and SRAP marker were more effective. To distinguish the 10 varieties, two-primer-combinations and three-primer-combinations were tested. Three-primer-combination BI26?PK1+PK2 and S91 or S92 can identify the 22 tomato varieties effectively and produces specific fingerprint of each varieties. More over, cluster analyses indicated that the relationship between varieties was very complicated, and the cluster analysis could not get the desired result with limited informative data.
出处
《中国农学通报》
CSCD
2005年第5期84-89,共6页
Chinese Agricultural Science Bulletin
基金
国家高技术研究发展计划(863计划)(批准号:2002AA244021)
项目和北京市科技计划项目"蔬菜种质改良中心建设及产业化开发"(合同编号:H022020130130)资助。
