摘要
随着功能基因组学的发展,表达序列标签(expressed sequence tags,ESTs)已经成为开发以PCR为基础的新型分子标记的重要资源。综述了植物功能基因组研究中出现的EST-SSR、CAPS、SNP、SRAP和TRAP等新型分子标记的基本原理和特点。这些标记具有其显著的优势,如开发简便、信息量高和通用性好等,尤其是由于它们来源于基因编码区(ORFs),因此具有很高的物种间通用性,并且其多态性与基因功能变异相关联。目前,这些功能基因分子标记已广泛应用于遗传图谱构建、重要性状基因定位、比较作图、遗传多样性和品种鉴别、分子标记辅助选择育种等研究中。简要介绍了近年来所开展的功能基因分子标记工作,并说明了在使用这些功能基因分子标记时可能会出现的问题。
With the development of functional genomics, expressed sequence tags （ESTs）has become an important resource of PCR-based molecular markers for plants. The advancement in functional molecular markers for plants in the functional genomics era, including EST-SSR, CAPS, SNP, SRAP and TRAP were reviewed. Compared with conventional markers, the EST-derived markers are a novel type of molecular tool with remarkable advantages, such as being easily to develop, more informative, and highly transferable, especially for preferential targeting ORFs. Applications of the new functional molecular markers in plants genetics and summarized, such as construction of genetic linkage breeding are maps, important trait gene tagging location, comparative mapplng,genetic diversity,euhivars identification and marker-assisted selection breeding
出处
《中国生物工程杂志》
CAS
CSCD
北大核心
2007年第8期104-110,共7页
China Biotechnology
基金
国家"863"计划资助项目(2006AA100108-3-7
2006AA10Z123)
