摘要
目的通过UFLC-QTOF-MS技术,评价通脉颗粒的质量。方法对10个生产企业的61批次的通脉颗粒,采用Agilent proshell 120(100 mm×3.0 mm,2.7μm),流动相是甲醇-缓冲盐,梯度洗脱,体积流量0.4 m L/min,柱温40℃,ESI源,正离子模式下采集质谱数据。应用Markerview软件进行主成分分析(PCA)对通脉颗粒进行分组。结果正离子模式时PCA分析能很好的区分通脉颗粒的质量,通过t检验找出了大豆素8-C-芹糖(1,6)-O-葡萄糖苷和1,5-Anhydro-1-[5,7-dimethoxy-2-(4-methoxyphenyl)-4-oxo-4H-chromen-8-yl]hexitol两个差异最大的化合物。结论 PCA分析可以作为评价通脉颗粒质量的分类方法。
Objective To evaluate the quality of Tongmai Granule by UFLC-QTOF-MS. Methods The UFLC was performed on Agilent proshell 120 column （100 mm × 3.0 mm, 2.7 μm） with the mobile phase of MeOH- buffer salt （43：57） by gradient elution, the flow rate was 0.4 mL/min, the column temperature was 40 ℃, electrospray ionization source was used under the positive ion mode. Tongrnai Granule was classified by principle component analysis （PCA） with Markerview software. Results PCA analysis could distinguish the quality of Tongmai Granule under positive ion mode. Daidzein 8-C-apiosyl（1,6）-O-glucoside and 1,5-anhydro-1-[5,7-dimethoxy-2-（4-methoxyphenyl）-4-oxo-4H-chromen-8-y1]hexitol were found to be the biggest different compounds by t test. Conclusion PCA is an effective classification method to evaluate the quality of Tongmai Granule.
出处
《药物评价研究》
CAS
2016年第4期603-607,共5页
Drug Evaluation Research
基金
辽宁省自然科学基金优秀人才培养项目(2015020698)
