摘要
目的探讨旱莲草对海马内注射Aβ1-40诱导的阿尔兹海默病(Alzheimer’s disease,AD)模型大鼠认知和炎症介导因子(IL-1β、IL-6和TNF-α)表达的影响。方法 SD大鼠随机分为对照组、模型组、旱莲草低剂量组、旱莲草高剂量组。右侧海马注射Aβ1-40构建AD模型大鼠,同时每天灌胃给予低剂量(8 g/kg)或高剂量(24 g/kg)的旱莲草,持续8周。Y迷宫测试大鼠的认知功能;酶联免疫法(enzyme-linked immuno sorbent assay,ELISA)检测IL-1β、IL-6和TNF-α的变化;HE染色观察大鼠前额叶皮质细胞形态改变。计量资料比较采用单因素方差分析,组间两两比较采用LSD-t检验,P<0.05为差异有统计学意义。结果四组大鼠电击总数[(35.08±10.40)、(54.36±9.41)、(44.18±8.76)、(36.17±8.67)次]、出错总数[(12.33±4.27)、(24.36±5.87)、(19.09±4.48)、(13.83±5.67)次]、记忆出错总数[(9.83±3.16)、(16.54±3.45)、(13.27±3.04)、(10.67±2.02)次]比较,差异均有统计学意义(均P<0.05)。AD模型组大鼠电击总数、出错总数及记忆出错总数均较对照组明显增多,差异均有统计学意义(均P<0.05)。四组大鼠IL-1β[(52.67±3.12)、(75.92±5.39)、(63.82±7.98)、(56.11±6.27)pg/ml]、IL-6[(43.04±6.17)、(67.68±7.52)、(52.71±5.76)、(46.78±4.08)pg/ml]、TNF-α[(82.55±6.02)、(137.08±9.40)、(100.12±8.47)、(87.03±6.56)pg/ml]水平比较,差异均有统计学意义(均P<0.05)。结论旱莲草可以改善AD大鼠的认知功能障碍,减轻脑组织的病理损伤,其机制可能与降低AD大鼠血清IL-1β、IL-6、TNF-α的过度表达有关。
Objective To explore the effect of Eelipta prostrata on cognitive function and the expression of inflammatory mediators （IL-1β, IL-6 and TNF-α） in Alzhcimer＇ s disease（AD） model rats induced by injection of A β 1-40 in hippocampus. Methods SD rats were randomly divided into control group, model group, low-dose Eclipta prostrata group, high-dose Eelipta prostrata group. AD model rats were established by injection of A β1-40 in right hippoeampus. Meantime, the rats were daily treated by intragastric administration of low doses （8 g/kg） and high doses （24 g/kg） of water extract of Eclipta prostrata for 8 weeks. After the treatment, the cognitive function was examined by using Y Maze test, the expression of IL-1 β, IL-6 and TNF- α was detected by ELISA assay, the morphological changes of prefrontal cortex were observed in HE staining. Measurement data was processed by variance analysis, pairwise comparison was used by LSD. P〈0.05 was considered statistically significant. Results Y Maze test showed that there were significant differences in the total number of electric shocks [（35.08±10.40）, （54.36 ±9.41）, （44.18 ± 8.76）, （36.17± 8.67） times], the errors to reach the learning standard [（12.33 ± 4.27）, （24.36 ± 5.87）, （19.09 ± 4.48）, （13.83 ± 5.67） times] and the errors of memory test [（9.83 ± 3.16）, （16.54± 3.45）, （13.27± 3.04）, （10.67± 2.02） times] of four groups （all P〈0.05）. Compared with control group, the total number of electric shocks, the errors to reach the learning standard and the errors of memory test in AD model rats sigrrificantly increased（all P〈0.05）. ELISA results showed that there were significant differences in the level of IL-1 β [（52.67 ± 3.12）, （75.92 ± 5.39）, （63.82 ± 7.98）, （56.11± 6.27）pg/ml], IL-6 [（43.04 ±6.17）, （67.68 ± 7.52）, （52.71 ± 5.76）, （46.78 ± 4.08） pg/ml] and TNF-α [（82.55 ± 6.02）, （137.08 ± 9.40）, （100.12 ± 8.47）, （87.03 ± 6.
出处
《社区医学杂志》
2016年第16期1-4,共4页
Journal Of Community Medicine
基金
河南省重点科技攻关项目(13210231 0263)
