摘要
目的观察小鼠高强度噪声短时暴露后,其行为学的改变,探索噪声对小鼠行为和听力的影响及机制。方法选取生后15 d的C57小鼠,随机分为两组,噪声暴露组30只,123 dB SPL白噪声固定时间暴露2 h;对照组30只,正常喂养,2个月后,检测ABR反应阈,将两组小鼠根据是否给予MWM训练再分为两个亚组,剥离小鼠海马,检测氧化应激水平,实时荧光定量PCR法测定各组小鼠海马组织中即刻早基因IEGs的表达水平。结果强噪声单次暴露后2个月,与对照组比较,噪声暴露组动物ABR反应阈升高,小鼠的Morris水迷宫行为表现显著改变,差异有统计学意义(P<0.05);两组小鼠海马组织中氧化应激水平比较,差异无统计学意义(P>0.05)。小鼠海马组织中即刻早基因例如Npas4、Arc的表达量可通过学习与训练来上调,但噪声暴露组动物通过学习训练致使两基因上调的变化低于对照组(P<0.05)。结论高强度噪声短时暴露对小鼠的行为和听力有负性影响。学习训练可显著上调小鼠海马组织中即刻早基因的表达,而噪声暴露的作用在统计学上无显著性差异,这可能是噪声影响学习记忆能力的分子机制之一。
Objective To observe the behavioral changes of mice exposed to high intensity noise for a short time,and to investigate the impacts of noise on behavior and hearing of mice and its mechanism.Methods C57 mice of 15 days after birth were randomly divided into the noise exposure group(n=30)and the control group(n=30).The noise exposure group was exposed to 123 dB SPL white noise for 2 hours,while the control group was fed normally.After 2 months,the ABR response threshold was detected.The two groups of mice were divided into two subgroups according to whether they were given MWM training or not.The hippocampus of mice was stripped,and the oxidative stress level was detected.The expression level of immediate early genes(IEGs)in hippocampus of mice in each group was determined by real-time fluorescence quantitative PCR method.Results Two months after single exposure to strong noise,compared with the control group,the ABR threshold of animals in the noise exposure group was increased,and the Morris water maze behavior of mice was changed significantly,with statistically significant difference(P<0.05).There was no significant difference in oxidative stress level between the two groups of mice(P>0.05).The expression amount of IEGs such as Npas4 and Arc in hippocampus of mice could be up-regulated by learning and training,but the changes of up-regulation of the two genes in the noise exposure group were lower than those in the control group through learning and training(P<0.05).Conclusion Short-term exposure to high intensity noise has negative impacts on the behavior and hearing of mice.Learning and training can significantly up-regulate the expression of IEGs in hippocampus of mice,while the effect of noise exposure has no statistically significant difference,which may be one of the molecular mechanisms of noise impacting learning and memory ability.
作者
陶珊
刘秀平
卓志强
TAO Shan;LIU Xiuping;ZHUO Zhiqiang(Department of Special Needs,Children′s Hospital of Fudan University Xiamen Branch,Xiamen Children′s Hospital,Xiamen 361006,China;Department of Respiratory,Children′s Hospital of Fudan University Xiamen Branch,Xiamen Children′s Hospital,Xiamen 361006,China)
出处
《中国现代医生》
2020年第34期44-47,51,共5页
China Modern Doctor
基金
福建省厦门市儿童医院青年科研项目(CHP-2019-YRF-0007)。
关键词
噪声
认知功能
氧化应激
海马
即刻早基因
Noise
Cognitive function
Oxidative stress
Hippocampus
Immediate early genes
