摘要
目的研究输血相关急性肺损伤(TRALI)大鼠肺组织细胞因子诱导的中性粒细胞趋化因子1(CINC-1)mRNA表达水平的变化,探讨CINC-1在大鼠TRALI发病机制中的作用。方法将60只SD大鼠随机分为TRALI组、正常对照组、脂多糖(LPS)对照组和阳性对照组,每组15只大鼠。LPS对照组大鼠经腹腔注射LPS(2 mg/kg,1 min内完毕),2 h后静脉输注1 m L生理盐水;TRALI组大鼠经腹腔注射LPS(2 mg/kg)2 h后静脉输注1 m L储存28 d的同种异体血浆;正常对照组大鼠采用假手术处理;阳性对照组大鼠移除约等于大鼠10%血容量的血液(1 m L)后,经静脉输注LPS(5 mg/kg)诱导急性肺损伤。采用逆转录聚合酶链反应检测大鼠肺组织中CINC-1 mRNA表达水平,采用酶联免疫吸附试验测定肺组织匀浆CINC-1蛋白含量;检测肺湿/干重比、肺组织髓过氧化物酶(MPO)活性,观察肺组织病理学改变,比较支气管肺泡灌洗液(BALF)中细胞总数和中性粒细胞百分比。结果与正常对照组和LPS对照组比较,TRALI组肺组织中CINC-1蛋白和CINC-1 mRNA表达明显增高[(18.98±2.21)ng/g和[(26.42±3.37)ng/g比(38.95±4.32)ng/g,F=11.28,P<0.05;(0.24±0.09)和(0.15±0.08)比(0.38±0.09),F=15.31,P<0.05]。TRALI组BALF中细胞总数和中性粒细胞百分比[(310.63±76.67)×106/L和(33.57±11.51)%]明显高于正常对照组[(101.36±63.83)×106/L和(9.87±3.56)%](F=13.82、5.41,P<0.05)。TRALI组肺组织含水量和MPO活性明显高于正常对照组(F=5.78、13.46,P<0.05)。阳性对照组各指标均显著高于TRALI组(P<0.05)。结论 TRALI大鼠肺组织中CINC-1的表达升高,肺组织中性粒细胞浸润增多,提示CINC-1参与了中性粒细胞与内皮细胞的粘附和聚集,推测CINC-1可能在TRALI的发病过程中起着重要作用。
Objective To study the expression of cytokine-induced neutrophil chemoattractant-1（CINC-1） in rats with transfusion-related acute lung injury（ TRALI）,explore its possible role in the pathogenesis of TRALI. Methods Sixty Sprague-Dawley rats were randomly divided into a normal control group with sham operation,a positive control group with ALI induced by intravenous infusion of lipopolysaccharide（5 mg / kg）,and a TRALI group treated by intraperitoneal injection of LPS 2h before the transfusion of human plasma（ 1m L）,a LPS control group treated by intraperitoneal injection of LPS 2h before the transfusion of normal saline（ 1m L）. The reverse transcription-polymerase chain（ RT-PCR） was used to detect CINC-1 mRNA. The level of CINC-1 in lung tissue homogenate was measured by ELISA.Morphological changes of the lung tissue were observed under light microscope. Myeloperoxidase（ MPO） in lung homogenate and wet lung weight to dry lung weight ratio（ W / D） were observed. The number of cells and the percentage of polymorphonuclear neutrophil（ PMN） in Bronchoalveolar lavage fluid（ BALF） were also compared. Results Compared with the normal control group and the LPS control group,the expression of CINC-1 protein and CINC-1 mRNA were increased significantly in lung of the positive control group and the TRALI group（ P 〈 0. 05）. The number of cells and the percentage of PMN in BALF of the TRALI group[（310. 63 ± 76. 67） × 106/ L and（33. 57 ± 11. 51） %] were significantly higher than those in BALF of the normal control group [（101. 36 ± 63. 83） × 106/ L and（9. 87 ± 3. 56） % ]（ P 〈 0. 05）. Tissue water content and MPO activity in the TRALI group were significantly higher than those in the normal control group（ P 〈0. 05）. Conclusion Expression of CINC-1 protein and CINC-1 mRNA are increased in the rat lung with TRALI and PMN infiltration in lung tissue,which suggests CINC-1 participate in the process of the PMN and endothelial cell adhesion and may play an
出处
《中国呼吸与危重监护杂志》
CAS
北大核心
2016年第4期379-383,共5页
Chinese Journal of Respiratory and Critical Care Medicine
