摘要
背景:在脑缺血后神经功能的恢复过程中,突触素可以发挥出至关重要的作用。目的:分析骨髓间充质干细胞移植后脑梗死大鼠神经功能改变以及与突触素表达的关系。方法:60只大鼠随机分为4组,每组15只,分别设为假手术组和对照组、模型组、干细胞治疗组。对照组、模型组、干细胞治疗组均制备脑梗死模型,假手术组实施假手术操作。造模后1 d,干细胞治疗组侧脑室移植骨髓间充质干细胞治疗,对照组注射相同剂量的PBS。治疗后1,7,14 d分别对各组动物进行平衡木行走实验、转棒上行走实验、网屏实验评分和RT-PCR检测以及免疫组织化学检测突触素表达。结果与结论:(1)治疗后7 d和14 d,干细胞治疗组平衡木行走实验、转棒上行走实验、网屏实验评分均显著低于对照组和模型组(均P<0.05),对照组、模型组之间比较差异无显著性意义(P>0.05);(2)治疗后1 d,假手术组的突触素m RNA表达和突触素阳性细胞数均显著高于其他3组(均P<0.05),治疗后7 d和14 d,干细胞治疗组的突触素mR NA表达和突触素阳性细胞数均显著高于其他3组(均P<0.05),且假手术组的突触素mR NA表达和突触素阳性细胞数均显著低于模型组和对照组(均P<0.05);(3)结果提示,骨髓间充质干细胞移植治疗可以有效促进脑梗死大鼠神经功能的恢复,并促进局部突触素的生成。
BACKGROUND：Synaptophysin plays an important role in the recovery of neural function after cerebral ischemia. OBJECTIVE：To investigate the effects of bone marrow mesenchymal stem cel transplantation on nervous function and expression of synaptophysin after cerebral infarction. METHODS：Total y 60 rats were equivalently randomized into four groups, including sham operation, control, model and stem cel treatment groups. Rats in the control, model and stem cel treatment groups were used for preparing cerebral infarction models, and the remaining underwent the sham operation. After 1 day of modeling, bone marrow mesenchymal stem cel s were transplanted into the rat lateral ventricle in the stem cel treatment group, and rats in the control group was given the injection of the same amount of PBS. After 1, 7 and 14 days of treatment, rat’s neurological function was scored on beam-walking test, rotarod test and screen test, and expression of synaptophysin was detected by RT-PCR and immunohistochemical assay. RESULTS AND CONCLUSION：At 7 and 14 days after treatment, the beam-walking test, rotarod test and screen test scores in the stem cell treatment group were significantly lower than those in the control and model groups （P〈0.05）, and the above scores showed no significant differences between the control group and model group （P〉0.05）. At 1 day after treatment, the mRNA expression of synaptophysin and the number of synaptophysin-positive cel s in the sham operation group were significantly higher than those in the other three groups （P〈0.05）;at 7 and 14 days after treatment, the mRNA expression of synaptophysin and the number of synaptophysin-positive cells in the stem cell treatment group were significantly increased compared with the other three groups （P〈0.05）, and additionally, the mRNA expression of synaptophysin and the number of synaptophysin-positive cells in the sham operation group were significantly lower than those in the model and control groups （P〈0.05）. These findings
出处
《中国组织工程研究》
CAS
北大核心
2016年第28期4182-4188,共7页
Chinese Journal of Tissue Engineering Research
