摘要
目的研究黄芪皂甙Ⅳ联合骨髓间充质干细胞(bone mesenchymal stem cell,BMSCs)移植对大鼠脑缺血再灌注模型血管生成和神经功能的影响。方法采用线栓法建立大鼠大脑中动脉缺血模型,随机分为模型组、黄芪皂甙Ⅳ组、BMSCs组、黄芪加BMSCs组(联合组),应用PKH-26染色标记移植的BMSCs,NSS法检测神经功能恢复情况,免疫组化染色检测基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)和血管内皮生长因子(vascular endothelial growth factor,VEGF)蛋白表达,CD34标记血管内皮细胞并计数微血管密度(MVD)。结果与模型组和黄芪皂甙Ⅳ组比较,黄芪加BMSCs组和BMSCs组均能使大鼠神经功能损害评分降低,以黄芪加BMSCs组恢复最为明显(P<0.05)。与模型组比较,黄芪皂甙Ⅳ组SDF-1蛋白表达显著增加(P<0.05)。黄芪加BMSCs组PKH-26标记的细胞个数显著高于BMSCs组(P<0.05)。治疗组VEGF和MVD表达均显著高于模型组,其中黄芪加BMSCs组表达最为显著(P<0.05)。结论联合组促进脑缺血损伤血管生成和神经功能恢复的作用优于单独治疗组,其机制可能与黄芪皂甙Ⅳ上调脑缺血区SDF-1表达,促进移植的BMSCs迁移和存活有关。
Objective To investigate the effects of astragaloside Ⅳcombined with bone mesenchymal stem cell on neurological outcome and angiogenesis after cerebral ischemia-reperfusion in rats.Methods The rat model of middle cerebral artery occlusion was established in model,SD rats were randomly divided into model group,BMSCs group,astragaloside Ⅳ group and astragaloside Ⅳ＋BMSCs group.Neurological function score were investigated.The expressions of SDF-1 and VEGF proteins were detected by using immunohistochemical method.MVD was evaluated by immunohistochemistry with the specific antibody CD34.Results Compared with model group and astragaloside Ⅳ group,the neurological scores of astragaloside Ⅳ＋ BMSCs group and BMSCs group were significantly decreased,a significant discrepancy also existed between astragaloside ＋ BMSCs group and BMSCs group（P〈0.05）.Compared with model group,the expression of SDF-1 proteins was significantly increased in astragaloside Ⅳ group（P〈0.05）.The number of PKH-26 labeled BMSCs in astragaloside ＋ BMSCs group was higher than in BMSCs group（P〈0.05）.Compared with model group,the expression of VEGF and MVD were significantly increased in treated group.moreover,the effect of astragaloside Ⅳ ＋ BMSCs group was most powerful among treated group（P〈0.05）.Conclusion The combined treatment promoted functional outcome and angiogenesis significantly more than either of the single treatments after cerebral ischemia reperfusion,which may be related to astragaloside IV upregulating SDF-1 expression in the ischemic brain,promoting BMSC survival and migration.
出处
《解剖学研究》
CAS
2011年第5期323-326,343,共5页
Anatomy Research
基金
黑龙江省普通高等学校青年学术骨干支持计划项目(1152G050)
黑龙江省卫生厅科研项目(2010243)
