摘要
目的：目前对应用神经干细胞和骨髓基质干细胞移植治疗帕金森病效果进行对比的研究很少,实验观察比较同种异体来源的中脑神经干细胞和骨髓基质干细胞对帕金森大鼠行为学及损伤脑组织形态学的影响。方法：实验于2006-03/2007-09在河北医科大学第一医院脑老化与认知神经科学实验室完成。SD大鼠麻醉后建立右侧帕金森病模型,随机分为神经干细胞组14只、骨髓基质干细胞组10只、空白对照组10只。选取右侧纹状体2个坐标点（inmm：A＋0.6;R＋4.0;V-5.0）、（inmm：A-0.7;R＋3.0;V-5.0）,前两组每个坐标点分别注入经Brd-U标记的神经干细胞悬液、骨髓基质干细胞悬液5μL,约1×10^6个细胞,空白对照组注射等量磷酸盐缓冲液。细胞移植后各组大鼠腹腔注射阿朴吗啡诱导旋转。结果：①行为学改变：与空白对照组比较,移植2～8周神经干细胞组和骨髓基质干细胞组大鼠的旋转次数均明显减少（P〈0.01）。②纹状体切片免疫组织化学荧光染色鉴定：移植8周后,神经干细胞组和骨髓基质干细胞组均可见一定数量双标的神经元、星形胶质细胞和酪氨酸羟化酶阳性细胞,且后者的双标细胞相对多于前者。空白对照组未发现Brd-U阳性细胞、微管相关蛋白2阳性细胞及酪氨酸羟化酶阳性细胞的表达。各组损毁侧黑质酪氨酸羟化酶阳性细胞残存率基本相似（P〉0.05）。结论：神经干细胞和骨髓基质干细胞移植均可改善帕金森病大鼠的旋转行为,且至少在脑内存活8周,并可分化成神经元、星形胶质细胞和多巴胺能神经元。
AIM： Few researches are conducted to compare the therapy between neural stem cell （NSC） and bone marrow stromal cell （BMSC） transplantation for Parkinson disease. In this study, we compared the effect of allogenic intracerebral NSCs and BMSCs on the behavioral and histomorphological changes of rats with Parkinson disease. METHODS： The experiment was performed at the Brain Ageing and Cognitive Neuroscience Laboratory, First Hospital of Hebei Medical University from March2006 to September 2007. Models of Parkinson disease at right side were established in adult healthy female SD rats under anesthesia, and then were randomly divided into NSC group （n=14）, BMSC group （n=10）, and control group （n=10）. Two coordinate points were oriented in the right striatum （in ram： A ＋0.6; R ＋4.0; V -5.0） and （in ram： A -0.7; R ＋3.0; V -5.0）. NSC suspension （1×10^6/is L, 5 μL） and BMSC suspension （1×10^6/μL, 5 μL） labeled by Brd-U were injected stereotaxically into the right striatum of NSC and BMSC groups, respectively, while the control group was injected with the same dose of phosphate buffer solution. Apomorphine （APO） -induced rotation behavior was observed after transplantation. RESULTS： （1）Behavioral changes： Compared with control group, the APO-induced rotation times of rats in NSC group and BMSC group decreased significantly from 2^nd to 8^th week after transplantation （P 〈 0.01）. （2）Immunohistochemistry and immunofluoresence identification： Some double straining neurons, astrocytes and tyrosine hydroxylase-immunoreactive （TH-IR） cells were seen in striatum of NSC group and BMSC group 8 weeks after transplantation, and the double positive cells of BMSC group were more than that of NSC group. There was no expression of Brd-U-positive cell, microtubule-associated protein-2-positive cell and TH-IR cell in control group. The survival rates of TH-IR cells in the substantia nigra of lesion side in three groups were basically similar （P 〉 0.05�
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2008年第12期2221-2226,共6页
Journal of Clinical Rehabilitative Tissue Engineering Research
基金
国家自然科学基金(30400138)
河北省自然科学基金(C2004000583)~~
