摘要
为了提高红曲菌(Monascus purpureus)FM-4000的红色素色价,采用优化种龄和接种量的方法,探索了分批补料发酵基质对红色素色价的影响。结果表明,最佳种龄为6 h二级种子液,接种量10%(V/V),在发酵48 h时补加20 g/L黄豆粉,在发酵84 h时补加0.5 mol/L氨水,在发酵96 h时补加60 g/L米粉。在此工艺条件下,摇瓶发酵红曲菌FM-4000的红色素色价达到135.61 U/m L,相对初始摇瓶红曲菌FM-4000的红色素色价提高了37.25%;在5 L发酵罐中对红曲菌FM-4000进行分批补料发酵培养,红色素色价可达145 U/m L。
In order to improve the color value of red pigment produced by Monascus purpureus, the inoculum age and inoculum were optimized, and the effect of fed-batch fermentation substrate on the color value of red pigment was researched. Results showed that the optimum conditions were in- oculum age 6 h, inoculum 10% （V/V）, feeding 20 g/L soybean meal at fermentation 48 h, 0.5 mol/L aqueous ammonia at 84 h and 60 g/L rice flour at 96 h. Under the conditions, the color value of red pigment produced by M. purpureus FM-4000 in shake flask fermentation was up to 135.61 U/ml, which was 37.25% higher than that before optimization. The color value of red pigment was up to 145 U/ml after M. purpureus FM-4000 fed-batch fermentation in 5 L fermentor.
出处
《中国酿造》
CAS
北大核心
2016年第6期133-137,共5页
China Brewing
基金
国家文物局指南针计划项目(No.20130306)
关键词
红曲菌
红色素
分批补料发酵
Monascus purpureus
red pigment
fed-batch fermentation
