摘要
选择鼠伤寒沙门氏菌和甲型副伤寒沙门氏菌分别与检验中常见的5种其它肠杆菌属细菌组合成染菌虾仁模拟样品10组,按照现行的检验标准GB 4789.4-2010进行沙门氏菌检验^([1])。10组样品均能检出沙门氏菌和干扰菌,检验结果准确。在检验过程中发现BS平板需要经过培养48 h才能观察到明显的菌落特征;增菌液培养时间越长,沙门氏菌的生长越好;甲型副伤寒沙门氏菌有微弱产H_2S现象;粘质沙雷氏菌对鼠伤寒沙门氏菌的硫化氢反应有抑制作用;奇异变形杆菌和柠檬酸杆菌与沙门氏菌属A-F多价O诊断血清发生交叉凝集反应现象。培养过程中发现的检测技术问题,在水产品、动物及动物产品的沙门氏菌等肠杆菌属的检验中,都有很好的借鉴意义。
Combining the standard bacteria of Salmonella typhimurium and Salmonella paratyphi respectively with five other Enterobacter bacterium which are common in the usual determination to form into 10 groups of modal Bacteria-bearing Peeled Prawms,isolating and identifying them under the following present standards：GB 4789.4-2010,National food safety standard Food microbiological examination：Staphylococcus aureus. All the standard bacteria of Salmonella and the Enterobacter bacterium can be exactly detected from the 10 groups of test samples. The experiment shows that the characteristics of the colony on the BS would be more evident after culturing for 48 h.And the longer the enriched culture cultures,the better the Salmonella grows. Salmonella paratyphi produces a little of H2 S. Serratia marcescens inhibits the H2S-reaction of Salmonella paratyphi.Anti-O polyvalent serum of A-F of Salmonella can agglutinates Proteus mirabilis and Citrobacter spp. The determination techniques found in the test would be applied well in the determination of Salmonella for the aquatic products,animals and animal products.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2016年第5期556-559,共4页
Journal of Food Science and Biotechnology
