摘要
目的了解淮安市发热病人疟原虫血片质量,为镜检质量考核以及消除疟疾效果评价提供科学依据。方法对淮安市各县(区)疟疾中心镜检站已复检血片和辖区内门诊镜检站阴性血片进行随机抽样,抽取所有网络直报的阳性血片和不少于3%的阴性血涂片进行复检。从血片制作、染色和清洁度方面对血片质量进行对比分析和评价。结果共抽查已检血片5 086张,血片制作、染色和清洁度总体合格率分别为90.25%、92.24%和92.57%,不同年份复检血片质量差异均有统计学意义(Ρ<0.05),并有逐年增高的趋势。不同月份复检血片质量差异有统计学意义(Ρ<0.05)。不同县区复检血片质量差异有统计学意义(Ρ<0.05)。血片质量缺陷以沉渣、厚、薄血膜制作不规范以及偏碱性为主。结论淮安市疟原虫血片质量较好,达到省疟疾中心镜检站制定的判定标准。但仍需加强培训和督导,促进血片质量不断提高。
Objective To understand the quality of retested blood smears of Plasmodium in Huai'an City so as to providethe evidences for effective evaluation of malaria control and prevention. Methods All the positive blood smears ofPlasmodium and more than 3% negative ones were sampled yearly from different hospitals of Counties(Districts)in Huai'anCity. The comparative analysis and evaluation were performed based on the quality of sampled blood smears. Results A totalof 5 086 tested blood smears of Plasmodium were sampled. The total pass rates of blood smear making,dyeing and cleanlinesswere 90.25%,92.24% and 92.57%,respectively. There was an increasing trend year by year. The quality of blood smears wasstatistically significant among different years(P〈0.05),different months(P〈0.05)and different counties(P〈0.05). The defectsof blood smears mainly included sediment,non-standard thick film making and more alkaline. Conclusion In Huai'an City,the quality of retested blood smears is better and has achieved the check level formulated by the Provincial Microscopy Stationof Malaria. However,more training and supervision still should be strengthened in order to continue to improve the quality ofblood smears.
出处
《中国热带医学》
CAS
2016年第5期492-494,498,共4页
China Tropical Medicine
关键词
疟疾
疟原虫
血涂片
复检
Malaria
Plasmodium
Blood smear
Recheck