摘要
以8种不同基因型的萝卜为试验材料进行带柄子叶离体培养,筛选诱导不定芽和不定根的最佳培养基配方。结果表明:喜诺晓青和翠绿萝卜具有高频再生潜力,由其带柄子叶诱导不定芽的最佳培养基为:MS培养基+30 g·L-1蔗糖+0.7%琼脂+5 mg·L-16-BA+0.05 mg·L-1NAA,诱导不定根的最佳培养基为:MS培养基+30 g·L-1蔗糖+0.7%琼脂+5 mg·L-16-BA+0.10 mg·L-1NAA。
To select the best hormone combination for inducing adventitious shoots and adventitious roots,the in vitro culture of cotyledons with petiole from 8 genotypes of radish were carried out in this research. The results showed that Xinuoxiaoqing and Cuilü radish had high- frenquency regeneration potential. The optimum medium for their adventitious shoot induction was MS ＋ 30 g·L- 1Sucrose ＋ 0. 7% Agar ＋ 5 mg·L- 16- BA ＋ 0. 05 mg·L- 1NAA,and that for adventitious root induction was MS ＋ 30 g·L- 1Sucrose ＋ 0. 7% Agar ＋ 5 mg·L- 16- BA ＋0. 10 mg·L- 1NAA.
出处
《山东农业科学》
2016年第4期21-23,共3页
Shandong Agricultural Sciences
基金
"十二五"国家科技支撑计划项目(2012BAD02B01-6
2013BAD01B04-18)
山东省现代农业产业技术体系项目(SDAIT-02-022-01)
关键词
萝卜
愈伤组织
不定芽
不定根
激素
Radish
Callus
Adventitious shoot
Adventitious root
Hormone
