摘要
解析控制植物不定芽离体再生能力的遗传因素,有助于从根本上提高离体培养困难材料的不定芽再生能力,是植物基因工程遗传改良的基础。本研究以结球白菜4个纯合亲本及其杂交后代的带柄子叶为外植体,建立了高频率离体不定芽再生技术。据此我们还对白菜不定芽再生能力进行遗传分析,并开展亲本与后代杂种的离体培养及反应研究,包括对培养基的激素需求,不定芽形成频率,不定芽形成数量,芽形态等系列特征。研究结果表明,杂种与亲本在培养基的激素浓度上有类似需求;杂交后代的不定芽再生率均至少高于一方亲本,在一些组合中可以高于双亲;在适宜的杂交组合及培养条件下,结球白菜带柄子叶外植体的不定芽再生率可以达100%,每一外植体上的不定芽数达3～7个。方差分析表明,结球白菜的不定芽离体再生能力存在基因的加性效应。
Resolving the genetic factors of controlling plant adventitious shoot regeneration in vitro can improve the regeneration frequency of some recalcitrate species radically, which is the base of plant genetic engineering. In this research, we employed the cotyledonary petioles in Chinese cabbage of four parental lines and their crossed hybrids as explant, and established the techniques with a high shoot regeneration frequency in vitro. Based on these, we still studied the genetic analysis on the regenerating ability of Chinese cabbage and carried on the invastigation of in vitro cultivation and response of parental lines and their crossed hybrids, including the series of characteristics of requirement on the hormone concentration, the shoot regeneration frequency, the shoot morphology and shoot number per explant etc. The results demonstrated that there was a parallelism requirement on the hormone concentration in the medium between the parents and their hybrids; the shoot regeneration frequency of the hybrid was at least higher than one of the parents, and it was even obviously higher than both of the parents in some cross combinations. Furthermore, during the fitting cross combination and culture condition, the highest regeneration frequency of the cotyledonary petioles in Chinese cabbage could reach 100% and obtained 3-7 shoots per explant. Analysis of variance showed that the occurrence of additive gene effects in the in vitro shoot regeneration in Chinese cabbage.
出处
《基因组学与应用生物学》
CAS
CSCD
北大核心
2010年第2期332-338,共7页
Genomics and Applied Biology
基金
国家863项目(2008AA10Z154)
北京市科技合同项目(Z07070501770704)共同资助
关键词
结球白菜
外植体
不定芽再生
遗传分析
Chinese cabbage （Brassiea campestris L. ssp. pekinensis）, Explant, Shoot regeneration, Genetic analysis
