摘要
对一株源于海参肠道的季也蒙假丝酵母产胞外多糖进行分离纯化并对其抗氧化活性进行研究。采用乙醇沉淀、Sevag除蛋白等方法得到胞外粗多糖EPS。EPS经Sepharose强阴离子交换层析分离后,分别得到3个组分EPS1、EPS2和EPS3,对抗氧化活性较高的EPS2采用Sephacryal凝胶过滤层析进行纯化,得到1个单一组分EPS2-1,采用气相色谱法分析其单糖组成,并验证其抗氧化活性。结果表明:EPS2-1是由木糖、甘露糖、葡萄糖和半乳糖组成的;它具有较强的抗氧化活性,当其浓度为0.36 mg/m L时,对羟基自由基的清除率可达100%,明显高于同浓度下Vc对羟基自由基的清除率14.42%;当EPS2-1浓度为0.60 mg/m L时,对超氧阴离子自由基的清除率可达53.22%;同时EPS2-1表现出一定的还原力。研究证明该活性多糖具有很好的应用潜力,值得进一步研究开发。
In order to study the antioxidant activity in vitro,the exopolysaccharides were extracted from the culture of Candida guilliermind isolated from sea cucumber intestine. The fermentation liquor was treated with ethanol and sevag reagent to get the exopolysaccharides（ EPS）. Then three components（ EPS1,EPS2,EPS3） were separated by Sepharose anion exchange chromatography. The antioxidant activity of EPS2 was found more potent compared to the other two components. The purified exopolysaccharides（ EPS2-1） were obtained by Sephacryal chromatography. Gas chromatography was employed for the component analysis of EPS2-1 and the antioxidant activity of EPS2-1 was verified in vitro. The results indicated that EPS2-1 was composed of xylose,mannose,glucose and galactose. Moreover,EPS2-1possessed the strong antioxidant activity. The scavenging rate of · OH radical could reach to 100% when the EPS2-1concentration was 0. 36 mg / m L. This result was much higher than that of Vc（ 14. 42%） at the same concentration. When the EPS2-1 concentration reached to 0. 60 mg / m L,the scavenging rate of O-2·radical was about 53. 22%. Meanwhile,the EPS2-1 also had some reducing power. Based on above results,these active polysaccharides had more application potential for further study and development.
出处
《工业微生物》
CAS
CSCD
2016年第2期24-29,共6页
Industrial Microbiology
基金
辽宁省教育厅重点实验室(LZ2014029)
辽宁省研究生教育创新计划项目(2014-154)
国家海洋食品工程技术研究中心开放实验室(2012FU125X03)
关键词
季也蒙假丝酵母
胞外多糖
分离纯化
抗氧化活性
组成分析
Candida guilliermind
exopolysaccharides
isolation purification
antioxidant activity
component analysis
