摘要
以不同水分含量的新收获东北早粳稻稻谷作为研究对象,采用Illumina MiSeq高通量测序,分析二者真菌群落结构及多样性的差异,探究入仓储藏前不同水分稻谷真菌群落的原始状态。结果表明:根据97%的序列相似性水平,两种水分稻谷样品中的真菌群落共计属于2个门、26个纲、51个目、85个科、112个属、188个种。在门的水平上,两种水分的稻谷中真菌群落的优势菌门为子囊菌门Ascomycota和担子菌门Basidiomycota。在属的水平上,高水份稻谷中含量较高的为隐球菌属Cryptococcus 0.07%、Papiliotrema 3.81%,但在低水分稻谷中含量较低。通过Ven分析与PCA分析发现不同水分稻谷中尽管存在大量相同物种,但物种间丰富度存在差异,且高水分稻谷中存在的真菌菌种在种类和数量上要高于低水分稻谷。两组水分稻谷中具有显著差异的特征菌属如Filobasidium、Sarocladium、Mycosphaerella,对此可以通过降低稻谷水分来抑制其生长。综上,该研究对稻谷进入仓储前的水分控制提供了理论支撑。
To explore the original state of fungal community of rice with different water content before warehouse storage,Illumina MiSeq high-throughput sequencing was used to analyze the fungal community structure and diversity of newly harvested early japonica rice from Northeast China with different water content in this study.The result showed that the fungal communities in the two rice samples belonged to 2 phyla,26 classes,51 orders,85 families,112 genera and 188 species according to the 97%sequence similarity level.At the phylum level,the dominant fungal communities in rice with different water content were Ascomycota and Basidiomycota.At the genus level,the contents of Cryptococcus 0.07%and Papiliotrema 3.81%were relatively high in high-moisture rice and relatively low in low-moisture rice.It was found that there were a large number of the same species in rice with different water content,but there were differences among species richness.And the species and number of fungi in rice with high water content were higher than those in rice with low water content.Therefore,it could be concluded that the specific bacteria,such as Filobasidium,Sarocladium and Mycosphaerella,which have significant differences between the two groups of rice in water content,can be inhibited by reducing the rice water content.In conclusion,this study provides theoretical support for water control of paddy rice before entering storage.
作者
李欣蔚
LI Xinwei(Liaoning Grain Science Research Institute,Shenyang 110032,China)
出处
《现代食品科技》
CAS
北大核心
2021年第12期57-64,96,共9页
Modern Food Science and Technology
基金
中国博士后基金项目(254358)。
关键词
稻谷
真菌
菌群结构
多样性
rice
fungi
microbial community structure
diversity
