摘要
目的观察头穴丛刺对阿尔茨海默病(AD)大鼠神经细胞凋亡的影响。方法 48只清洁级Wistar大鼠随机分为假手术组、模型组、治疗组、对照组各12只。采用Aβ1-42寡聚体进行造模,造模成功后,治疗组采用头穴丛刺治疗,对照组采用传统头针治疗,模型组与假手术组不进行任何手段干预。1次/d,6 d为1个疗程,间隔1 d,治疗4个疗程后,通过免疫组化方法测定海马区bcl-2、bax、caspase-3表达的变化。结果治疗组、对照组海马区bax、caspase-3表达较模型组均显著下降(P<0.01),治疗组表达低于对照组(P<0.05),bcl-2阳性神经元计数较模型组显著升高(P<0.01),治疗组表达高于对照组(P<0.05)。结论头穴丛刺能有效抑制AD大鼠海马区神经细胞凋亡进而提高认知水平。
Objective To observe the effect of cluster needling of scalp point therapy on neuron apoptosis of Alzheimer＇s disease rats. Methods Forty eight wistar rats were randomized into four groups including model group,sham operation group,observation group and control group. Establish model with Alzheimer ＇s disease by injecting Aβ1-42 oligomer into bilateral hippocampus. After successful modeling,the observation group was intervened by cluster needling of scalp point,the control group only received traditional needling of scalp,and the model group and the sham operation group took no treatment. All rats were treated once a day. One course of treatment was comprised of six days and one day interval. After four courses of treatment,observe the changes of bcl-2,bax and caspase-3 by immunohistochemistry. Results Compared with the model group,the expression of bax and caspase-3 of the observation group and the control group decreased significantly（ P〈0. 01）. While,there was a lower expression in the observation group than in the control group（ P〈0. 05）. Compared with the model group,the expression of bcl-2 of the observation group and the control group increased significantly（ P〈0. 01）. While,there was a higher expression in the observation group than in the control group（ P〈0. 05）. Conclusion The cluster needling of scalp point therapy could improve cognitive level by effectively inhibiting neuron apoptosis in hippocampus area of Alzheimer＇s disease rats.
出处
《长春中医药大学学报》
2016年第2期245-247,共3页
Journal of Changchun University of Chinese Medicine
基金
黑龙江省自然科学基金项目(H201476)
黑龙江省博士后流动站资助基金"头穴丛刺对Aβ1-40致AD大鼠MAPK级联信号通路影响的研究"(LBH-Z14199)
关键词
阿尔茨海默病
针灸疗法
头穴丛刺
神经细胞凋亡
Alzheimer＇s disease
acupuncture therapy
cluster needling of scalp point therapy
neuron apoptosis
