摘要
目的探讨香菜挥发油对Saos-2肿瘤细胞株体外生长及迁移的影响。方法采用超声波辅助醇提法制备香菜挥发油;MTT法检测香菜挥发油对Saos-2细胞生长抑制作用;Transwell趋化小室检测细胞迁移抑制作用;定量PCR检测细胞内P21 Cip1/Waf1、P27 Kip1、NM23H1和S100A4的表达变化。结果使用超声辅助醇提法,香菜挥发油得率为0.424%;香菜挥发油可明显抑制Saos-2细胞的生长与迁移,伴随细胞内P21Cip1/Waf1及P27 Kip1的升高及S100A4降低,显示具有较强的抗成骨肉瘤活性。结论香菜挥发油可体外抑制Saos-2细胞生长及迁移,其可能机制与P21 Cip1/Waf1、P27 Kip1及S100A4有关。
Objective To extract coriander volatile oil and investigate its effects on growth and migration of human Saos-2 cells in vitro. Methods Ultrasound-assisted ethanol extraction was used to prepare coriander volatile oil. MTT assay was used to determine the growth inhibion of Saos-2 cells by volatile oil. Cell migration was examined using Boyden chamber assay. Expression of P21 Cip1/Waf1, P27 Kip1, NM23H1 and S100A4 were examined by real-time PCR. Results The results showed that the yield of coriander volatile oil was 0.424% using ultrasound-assisted ethanol extraction. Coriander volatile oil inhibited the growth and migration of Saos-2 cells, along with the decrease in the expression levels of P21 Cip1/Waf1, P27 Kip1 and S100A4. Conclusions Coriander volatile oil can inhibit Saos-2 cell growth and migration in vitro, which may involve P21 Cip1/Waf1, P27 Kip1 and S100A4.
出处
《中国现代医学杂志》
CAS
北大核心
2016年第5期17-21,共5页
China Journal of Modern Medicine
基金
江西省卫生厅科技计划项目(No:20157101)
江西省教育厅科学技术研究项目(赣教技字GJJ08441)
