摘要
主要研究硅胶吸附L-α-GPC的动力学和热力学特性对吸附过程的控制,发现界面扩散控制速率模型1-3(1-X)2/3+2(1-X)能很好的拟合硅胶的吸附过程。通过Freundlich方程和Langmuir方程对吸附平衡模拟,得到了Langmiur方程拟合硅胶吸附L-α-GPC的吸附等温线,计算出不同温度条件下的回归方程,相关系数均在0.99以上,并证实了硅胶的吸附为放热反应。同时,利用Clausius-Clapeyron方程计算出3个吸附量的硅胶吸附热力学参数分别为80、160和240 mg/g,焓变(ΔHAm)分别为24.85、23.82、22.72 k J/mol。研究结果为进一步探讨工业化硅胶柱色谱制备高纯L-α-GPC提供了基础数据和理论参考。
The control of adsorption process of silica gel on the kinetics and thermodynamic of adsorption of L-alpha glycerylphosphorylcholine（ L- α- GPC） was studied. The results showed that the adsorption process of silica gel can be well governed by interface diffusion control rate model 1- 3（ 1- X）2 /3＋ 2（ 1- X）. The adsorption isotherm of L- α- GPC by silica gel could be described with the equation of Langmiur through the stimulation of adsorption balance by Freundlich and Langmuir equations,and then regression equation under different temperature conditions was calculated（ related coefficient was above 0. 99）,and silica gel adsorption was identified as exothermic reaction. The silica gel adsorption thermodynamic parameters of three absorption measurements were 80,160 and 240 mg / g respectively by Clausius- Clapeyron equation,and the adsorption enthalpy change（ ΔHAm） was 24. 85,23. 82,22. 72,respectively. The results provided the basic data and theoretical support for further exploration on the industrialized preparation of high purity L- α- GPC by silica gel column chromatography.
出处
《中国粮油学报》
EI
CAS
CSCD
北大核心
2016年第3期47-52,共6页
Journal of the Chinese Cereals and Oils Association
基金
国家自然科学基金(31301502)
河南省农业科学院优秀青年科技基金(2013YQ25)
关键词
甘油磷脂酰胆碱
硅胶柱色谱
动力学
热力学参数
L-alpha glycerylphosphorylcholine
silica gel column chromatography
kinetics
thermodynamic parament
