摘要
本研究建立了高效液相色谱-蒸发光散射法酶催化水解磷脂样品中的甘油磷脂酰胆碱(GPC)的方法。检测条件为:使用Alltech Silica色谱柱(4.6 mm×250 mm,5μm),柱温40℃,甲醇为流动相,流速1.0 mL/min,蒸发光散射检测器漂移管温度65℃,空气流速1.7 L/min。GPC为最后出峰的强极性组分,其保留时间为4.97 min,所得GPC标准曲线为Y=2.048 3 X+7.774 4,线性相关系数R2为0.991 4。检测限为6.3μg/mL(S/N=3),定量限为13.0μg/mL(S/N=10)。GPC的平均加标回收率为102.5%,RSD是2.2%(n=3),分析时间小于6 min。结果表明本方法分离效果好,检测结果准确、稳定,适用于酶催化水解磷脂样品中甘油磷脂酰胆碱的定量测定。
A HPLC-ELSD analysis method of glyrinphosphatidylcholine（GPC）in the samples of enzymatically hydrolyed lecithin was established in this research.The analysis conditions are as follows：Alltech Silica column（4.6 mm ×250 mm,5μm）,column temperature 40℃,methnol moving phase,flow rate 1 mL/min,drift tube temperature 65 ℃,and air flow rate1.7 mL/min.Because of the strong polarity,the peak of GPC is seen at last with retention time of 4.97 min.The standard curve of GPC is Y=2.048 3 X＋7.774 4（R2=0.991 4）,the limit of detection is 6.3μg/mL（S/N=3）,and the limit of quantitation is 13.0μg/mL（S/N=10）.The average standard recovery rate of GPC is 102.5%,RSD=2.2%（n=3）,and the time of analysis is less than6 min.This indicates that the HPLC-ESLD method can effectively separate the GPC in the spectroscopy and the analysis results are precise28 and stable.Therefore it＇s suitable for the quantification of glycerylphosphorylcholine which is made by enzymehydrolyze lecithin.
出处
《粮食与食品工业》
2014年第6期82-86,共5页
Cereal & Food Industry
基金
教育部新世纪人才项目(项目编号:NCET-12-0675)
广东省战略性新兴产业核心技术攻关项目(项目编号:2012A032300016)
广东省高新区发展引导专项(项目编号:2012B010900033)
