摘要
目的采用多重指标,优选长春胶囊的提取工艺,为其工业化生产提供依据。方法：采用紫外-可见分光光度法分别测定总皂苷、总黄酮及总多糖的含量。以总皂苷、总黄酮含量的综合评分为评价指标,采用L_9（3~4）正交试验法考察乙醇浓度、加醇倍量、提取时间对脂溶性成分提取工艺的影响;以总多糖含量为评价指标,采用L_9（3~4）正交试验法考察加水倍量、提取时间、提取次数对水溶性成分提取工艺的影响,优化长春胶囊的提取工艺。结果脂溶性成分提取的最佳工艺为10倍量65%乙醇回流提取三次,每次1.5h;水溶性成分提取的最佳工艺为8倍生药量水煎煮二次,每次1.5h。结论验证试验表明,该提取工艺稳定可行、重复性良好。研究为长春胶囊的工业化生产提供了依据。
AIM Using multiple indicators, optimizing extraction technology of changchun capsule and to provide basis for its industrialized production. METHODS： UV-visible spectrophotometry determination of total saponins, total flavonoids and total polysaccharides of. In total saponins, total flavonoids in the composite score for the index, using L_9（3~4） orthogonal experiment investigated ethanol concentration, the amount of ethanol, extraction time extraction process of fat-soluble ingredients; in total polysaccharide content evaluation, using L_9（3~4） orthogonal experiment study times the amount of water, extraction time, extraction times extraction of water-soluble components influence, optimization of extraction process Changchun capsules. RESULTS The optimum extraction of fat-soluble ingredients was 10 times the amount of 65% ethanol extracted three times,each time 1.5h;the optimum extraction of water-soluble components is 8 times the amount of crude drug boiling water twice, each time 1.5h.CONCLUSION： The experiment indicated that the extraction process is stable, good repeatability. Changchun capsule study provided the basis for industrial production.
出处
《人参研究》
2015年第4期16-19,共4页
Ginseng Research
基金
吉林省科学技术厅医药产业推进计划项目
项目编号:20140311049YY
关键词
长春胶囊
正交试验
提取工艺
Changchun Capsules
Orthogonal Design
Extraction Process
