摘要
目的 建立并评价抗CCP抗体、类风湿因子（RF）和C反应蛋白（CRP）对类风湿关节炎（RA）的最佳诊断界值。方法选择2014年6月～2015年3月以RA组130例、非RA组130例和对照组100例作为研究对象，采用酶联免疫吸附法测定血清抗CCP抗体，采用免疫透射比浊法测定血清RF及CRP的浓度，应用受试者工作特征曲线（ROC曲线）确定抗CCP抗体、RF和CRP的最佳诊断界值并评价三者的诊断效能。结果RA组和非RA组血清RF浓度分别为275．9±317．6和42．8±131．3IU／ml，前者显著高于后者（t=7．571，P〈0．05）；RA组和非RA组CRP浓度分别为39．2±47．3和29．7±42．2mg／L，前者显著高于后者（t=2．602，P=0．015）。抗CCP抗体、RF和CRP诊断RA的ROC曲线下面积（AUC）分别为0．868（0．820—0．916）、0．785（0．732～0．846）、0．587（0．512～0．661）。血清抗CCP抗体10RU／ml作为诊断界值时，诊断RA的敏感度、特异性和约登指数分别为0．669、0．892和0．562；RF以25IU／ml作为诊断界值时，诊断RA的敏感度、特异性、约登指数分别为0．715、0．777和0．492；CRP以30mg／L作为诊断界值时的敏感度、特异性和约登指数分别为0．469、0．623和0．092。结论抗CCP抗体、RF、CRP的对RA的最佳诊断界值分别为IORU／ml、25IU／ml和30mg／L。抗CCP抗体诊断效能高于RF，而RF诊断效能高于CRP。
Objective To establish and assess the cut - off values of anti - cyclic citrulIinated peptide antibody（anti - CCP）, rheumatoid factor（RF） and C - reactive protein（CRP） in serum for rheumatoid arthritis（RA）. Methods A total of 130 RA, 130 non - RA patients and 100 healthy people from June 2014 to March 2015 were enrolled. The concentration of anti - CCP was analyzed by ELISA, and the concentration of RF and CRP were analyzed by immunity transmission turbidity. The optimal diagnostic cut - off values of anti CCP, RF and CRP for RA were determined by ROC, and the diagnostic performance of them were assessed. Results The concentration of RF in RA and non - RA group were 275.9 ± 317.6IU/ml and 42.8 ± 131.3IU/ml respectively and the former was significantly higher than the latter. （t = 7. 571 ,P 〈 0.05 ）. The concentration of CRP in the RA and non RA group were 39.2± 47.3mg/L and 29.7 ±42. 2mg/L respectively and the former was significantly higher than the latter（ t = 2. 602,P = 0. 015 ）. The area under ROC curve （AUC） of anti - CCP, RF and CRP were 0. 868 （ 0. 820 - 0. 916）, 0. 785 （0. 732 - 0. 846）, 0. 587 （ 0.512 - 0.661 ）, respectively. For 10RU/ ml as cut - off value of anti - CCP, the sensitivity, specificity and Youden index was 0. 669, 0. 892 and 0. 562, respectively. For 25IU/ ml as cut - off value of RF, sensitivity, specificity and Youden index were 0. 715, 0. 777 and 0. 492, respectively. For 30mg/L as cut - off value of CRP, sensitivity, specificity and Youden index were 0. 469, 0. 623 and 0. 092, respectively. Conclusion The optimal diagnosis cut- off values of anti- CCP, RF and CRP were 10RU/ml, 25IU/ml, and 30rag/L, respectively. The diagnostic performance of anti - CCP was higher than that of RF,whicb was better than that of CRP.
出处
《医学研究杂志》
2016年第2期131-134,共4页
Journal of Medical Research
关键词
类风湿关节炎
抗CCP抗体
类风湿因子
C反应蛋白
Rheumatoid arthritis
Anti - cyclic citrullinated peptide antibody
Rheumatoid factor
C - reactive protein
