摘要
利用可口革囊星虫重组铁蛋白富集Pb^(2+)、Fe^(3+)、Cd^(2+)、Cr^(3+)、Cu^(2+)、Mn^(2+)、Sn^(2+)和Zn^(2+)等8种重金属离子后,通过透射显微镜和傅里叶红外光谱测定蛋白的超微结构变化和内部基团特性。以天然的马脾铁蛋白为对照,观察重组铁蛋白与天然铁蛋白的差别。结果显示,重组铁蛋白富集Pb2+和其它7种重金属离子后蛋白体积大小与重金属离子种类直接相关。重组铁蛋白相对天然铁蛋白来说在富集不同种类重金属离子后,除了非特异性酰胺特征峰和蛋白特征峰外,还出现一些专门富集某些重金属离子的特异基团,如:-CH_3、-CH_2-、-CH-、-NH-、-SO_3-、-COOH、-S=S-和-C-S-C等。通过细胞实验进一步验证了铁结合蛋白能够通过富集重金属离子Pb2+来保护小鼠成骨前体细胞MRC3T3-E1,使细胞的死亡率明显降低(P<0.05)。
The recombinant ferritin from Phascolosoma esculenta were exposed to the same concentration of different heavy metals to absorb them. The 8 kinds of heavy metals were Pb^2＋, Fe^3＋, Cd^2＋, Cr^3＋, Cu^2＋, Mn^2＋, Sn^2＋ and Zn^2＋. Transmission electron microscopy（TEM） and Fourier transform infrared spectroscopy（FTIR） were applied to study the ultra-structure and the change of groups structure. It compared to natural horse spleen ferritin with the same processing. The results indicated that the ultra-structure of ferritin was altered after trapping different kinds of heavy metals. Recombinant ferritin groups changed after trapping different heavy metals. There were some specific groups for binding heavy metals, including-CH3,-CH2-,-CH-,-NH-,-SO3-,-COOH,-S=S-,-C-S-C etc., besides some non-specific amides characteristic peaks and ferritin characteristic peaks. Further experiments verified that ferritin could protect the mouse osteoblast precursor cells MRC3T3-E1 through enrichment of Pb^2＋ and other heavy metal ions, and the cell mortality was significantly lower（P〈0.05）.
出处
《海洋与湖沼》
CAS
CSCD
北大核心
2015年第6期1358-1364,共7页
Oceanologia Et Limnologia Sinica
基金
国家自然科学基金资助项目
40776075号
41176123号
宁波大学研究生科研创新基金项目
G14042号
关键词
可口革囊星虫
重组铁蛋白
富集铅离子
超微结构
小鼠成骨前体细胞
Phascolosoma esculenta
recombinant ferritin
enrichment lead ion
ultra-structure
mouse osteoblast precursor cells
