摘要
用聚丙烯酰胺凝胶(PAGE)电泳法研究了镉对大弹涂鱼血细胞的乳酸脱氢酶(LDH)、过氧化物酶(POD)和超氧化物歧化酶(SOD)表达的影响。结果表明,在急性中毒条件下,和空白对照组相比,25、50和75mg/L三个处理组均有特异性酶带出现,各种酶活性也有显著变化。探讨了急性镉中毒对大弹涂鱼血细胞同工酶表达的影响,以及大弹涂鱼血细胞的同工酶标记用做海洋环境生态毒理学指标的可行性。
By method of polyarylamide gel electrophoresis, effect of cadmium on the patterns of three isozymes (LDH,POD and SOD) in blood cells of Boleophthalmus pectinirostris was revealed for the first time. The results showed that all the three concentration groups indicate differential patterns of the LDH,POD and SOD isozymes in the situation of acute toxicosis. And the activities of three isozymes showed significant variety. The influence of cadmium on the expression of isozymes in blood cells of Boleophthalmus pectinirostris and the feasibility of using isozymes markers as marine ecotoxicology indicators were discussed.
出处
《上海水产大学学报》
CAS
CSCD
2004年第4期289-292,共4页
Journal of Shanghai Fisheries University
关键词
镉
大弹涂鱼
血细胞
同工酶
生态毒理学指标
cadmium
Boleophthalmus pectinirostris
blood cells
isozymes
ecotoxicology indicators
