摘要
建立紫薇优良品种‘晓明1号’组培快繁体系,为大规模繁殖提供技术。以带腋芽茎段为外植体,对组培快繁体系中的基本培养基、生长调节剂浓度、组培苗移栽进行了研究,建立组培快繁体系。结果表明:带腋芽茎段接种在DKW+BA 1.0 mg/L+NAA 0.05 mg/L+PVP 300 mg/L的初代培养基上,腋芽萌发率95.56%,植株生长健壮;‘晓明1号’紫薇增殖培养及生根培养最佳配方为DKW+BA 1.0 mg/L+IBA 0.05 mg/L和1/2DKW+NAA 0.1 mg/L+AC 300 mg/L,增殖系数达到5.08,生根率达到100%,平均根条数6.2条,平均根长4.12 cm;用泥炭土:珍珠岩为2?1或3?1作为基质,以营养钵作为容器,组培苗移栽成活率达到95.33%以上,组培苗叶色鲜艳,长势良好。该研究获得了适合‘晓明1号’紫薇的组培方法,为品种的大规模繁殖和大面积推广提供了参考。
In order to establish a tissue culture and rapid propagation system, taking stems with axillary budsof Lagerstroemia indica‘Xiaoming 1＇as testing materials, basic medium, concentrations of plant growthregulators, proliferation and rooting stage, and the transplanting method were studied. The results showed thatthe medium DKW＋ BA 1.0 mg/L＋ NAA 0.05 mg/L＋ PVP 300 mg/L was suitable for inducing germination ofstem auxiliary buds, and the rate of germination was up to 95.56%. The best proliferation medium was DKW＋BA 1.0 mg/L＋ IBA 0.05 mg/L, and the multiplication coefficient was 5.08 with strong plantlets. The rootingpercentage, average root number and length per shoot reached 100%, 6.2 and 4.12 cm, respectively, on themedium 1/2DKW＋ NAA 0.1 mg/L＋ AC 300 mg/L. Taking nutrition pot as container, peat soil and perlite astransplanting medium（3：1 or 2：1）, the transplant survival rate was over 95.33%. There were many lateral rootsand the plantlets which grew rapidly and vigorously. This method was suitable for tissue culture of L. indica‘Xiaoming 1＇and laid foundations for its large-scaled propagation and large area promotion.
出处
《中国农学通报》
2016年第1期22-27,共6页
Chinese Agricultural Science Bulletin
基金
长沙市科技计划项目"大红高抗紫薇新品种高效繁育技术产业化开发"(K1406216-21)
关键词
紫薇'晓明1号’
组培
快繁体系
Lagerstroemia indica‘Xiaoming 1＇
tissue culture
rapid propagation system
