摘要
为保护濒危植物丹参,实现人工栽培,以嫩茎为材料,进行了愈伤组织诱导与分化、试管苗生根、继代、移栽和定植研究.结果证明:MS+6-BA0.3 mg.L-1+2,4-D2.0 mg.L-1+NAA0.8 mg.L-1是丹参嫩茎愈伤组织诱导培养和继代培养的理想培养基;1/2MS+AgNO30.2 mg.L-1和1/2MS+AgNO30.2 mg.L-1+6-BA 0.1 mg.L-12种培养基是丹参颗粒状愈伤组织块分化培养的理想培养基;以经过浓度为10mg.L-1的NAA溶液处理3 m in的不定芽为材料、以1/3MS+IAA0.4 mg.L-1为培养基的方法是丹参不定芽生根培养的理想方法.试管苗移栽成活率为92.7%,定植成活率为99.2%.定植的试管苗保持了野生丹参的所有生物性,建立起丹参的无性系.
The tender stem was used as materials to establish the rapid propagation technique of Salvia miltiorrhiza such as the calli induction and differentiation,rooting and transplanting of tube seedlings and so on.The results showed that the optimum culture medium for calli induction and subculture was MS+6-BA0.3 mg L-1+2,4-D2.0mg L-1+NAA0.8mg L-1;1/2MS+AgNO30.2mg L-1 and 1/2MS+AgNO30.2mg L-1+6-BA 0.1mg L-1 were the two kinds of optimum culture medium for differentiation of grainy calli induction.The adventitious buds could be easily induced rooting with the medium of 1/3MS +IAA0.4 mg L-1 after used NAA 10mg L-1,3min.The survival rate of transplanting to pot and field was 92.7% and 99.2% respectively.The tube seedlings' biological characters were same to the agrestal Salvia miltiorrhiza.
出处
《辽宁大学学报（自然科学版）》
CAS
2011年第3期214-218,共5页
Journal of Liaoning University：Natural Sciences Edition
关键词
丹参
组织培养
无性系
Salvia miltiorrhiza
tissue culture
clone
