摘要
以红叶紫薇当年生新枝具腋芽茎段为外植体材料,研究其灭菌时间长短对外植体污染率及腋芽萌发率的影响,根据基本培养基上其离体腋芽萌发诱导情况,对培养基(MS,1/2MS,WPM,1/2WPM)进行筛选,并根据植物生长调节剂(BA,NAA,KT)及其质量浓度的不同配合,筛选出无菌苗"一步法"增殖、生根培养基。试验结果显示:使用0.1%Hg Cl2对外植体灭菌12 min,能在保证低污染率(13.33%)的同时,获得较高的腋芽萌发率(80.00%);1/2MS培养基上的腋芽萌发率(96.67%)和腋芽长度(1.60 cm)均显著高于其他3种培养基;在1/2MS+1.0 mg/L BA+0.1mg/L KT培养基中,无菌芽可同时增殖及生根,芽增殖系数(4.67)、苗高(2.53 cm)、生根率(100%)及根数(4.33条)均显著高于其他处理。
The annual shoots of Red Crape Myrtle were used as explant for in vitro micro-propagation. Different sterilization manners,basal media and plant growth regulators were selected for one-step multiplication. The results showed that HgCl_2 sterilization for 12 min could keep explant in low contamination percentage but high germination rate. The germination rate（ up to 96. 67%） of axillary bud and bud length（ 1. 6 cm） of explant cultured on 1/2MS basal medium were significantly higher than those on MS,WPM and 1/2MS. The in vitro shoots could grow and multiply in one-step. Shoot length（ up to2. 53 cm）,root percentage（ 100%） and root number（ 4. 33） of in vitro shoots cultured on 1/2MS supplemented with 1. 0mg/L BA,0. 1 mg/L KT were significantly higher than those on other treatments. And the multiplication coefficient could also reach 4. 67.
出处
《江苏林业科技》
2017年第3期15-18,22,共5页
Journal of Jiangsu Forestry Science & Technology
基金
浙江省大学生新苗人才计划项目"红叶紫薇快繁关键技术的研究"(2016R412024)
关键词
红叶紫薇
快速繁殖
植物生长调节剂
Lagerstroemia indica Pink Velour
Rapid micro-propagation
Plant growth regulator
