摘要
为探索紫叶酢浆草的诱变方法,利用60Co γ射线诱变,研究处于分化态的紫叶酢浆草叶片诱导形成的再生体系。结果表明,处于分化态的紫叶酢浆草再生体系的愈伤组织不定芽、不定根的诱导数量随剂量的升高而降低。再生苗株高测量显示,10Gy的辐照剂量对再生苗株高的影响不显著,25Gy、50Gy的辐照剂量处理能显著抑制再生苗的株高,其中经50Gy辐照处理的再生苗株高仅为对照的36%。60Co γ射线照射对分化态的紫叶酢浆草再生体系诱发的变异类型主要有叶数、叶形、叶色等表型变异,其中以叶数变异为主(占变异总数的76%)。M2代表型变异频率为2.9%,表型变异仍以叶数变异为主。
To study the mutation method of Oxalis triangularis purpurea,the Oxalis triangularis purpurea regeneration system generated from the leaf was irradiated by 60Co γ-rays. It shows that the number of adventitious bud and root induced from the differentiation state Oxalis triangularis purpurea regeneration system are cut down with the increment of absorbed doses. After growth seedling length has not distinctly reduced while the absorbed dose is 10 Gy. And it has reduced at 25 Gy and 50 Gy of 60Co γ-rays irradiation with the differentiation state tissue of Oxalis triangularis purpurea. The mutation,such as leaf number,leaf colour and leaf shape mutation,mainly the leaf number mutation （76% of the total mutation） occur in the 60Co γ-rays irradiated group. The M2 mutation rate is 2.9%,and the main mutations are leaf number mutations.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
2010年第2期75-78,共4页
Journal of Radiation Research and Radiation Processing
基金
安徽省科技攻关(06013119B)
国际合作项目(09080703043)资助
关键词
Γ射线
紫叶酢浆草
分化态
γ-rays
Oxalis triangularis purpurea
Differentiation state
