摘要
以一株从新疆荒漠地区筛选出来的沙漠产油微藻为实验对象,经过形态学和分子生物学手段(18s rRNA及ITS测序)鉴定为链带藻属(Desmodesmus sp.),并命名为XJ842。为提高其油脂产率,采用响应面对其培养基组成(碳源、氮源和磷源)进行优化。单因素实验分析结果显示,其最适产油的碳源为Na2CO3,最适氮源为Na NO_3。运用Box-Behnken中心组合实验以及Design-Expert分析软件建立了以总脂含量和油脂产率为响应值的响应面方程,得到最适合产油的Na2CO3浓度为1.98 mmol/L,NaNO_3浓度为48.00 mmol/L,K_2HPO_4浓度为0.46 mmol/L。在最优培养基条件下,Desmodesmus sp.XJ842总脂含量达到67.73%,油脂产率达到29.27 mg/(L·d)。优化后的总脂含量、油脂产率比优化前的分别提高了2.08倍和2.61倍。优化后Desmodesmus sp.XJ842油中饱和脂肪酸和单不饱和脂肪酸占总脂肪酸的88.41%,且亚麻酸的含量小于12%,符合欧洲生物柴油标准EN14214,具有生产生物柴油的潜力。
An oil -rich desert microalgae isolated from desert regions in Xinjiang province was identified as Desmodesmus sp. by morphology and molecular biology （18s rRNA and ITS sequencing） and named XJ842. Its composition of culture medium （carbon source, nitrogen source and phosphorus source） was optimized to enhance its oil yield. The analysis results of the single factor experiment showed that the op- timal carbon source and nitrogen source were Na2CO3 and NaNO3 ,respectively. With the total oil content and oil yield as response values, the response surface equation was established by Box - Behnken center combination experiment combined with Design -Expert software. And the optimal concentrations of Na2CO3 , NaNO3 and K2HPO4 were 1.98,48.00 mmol/L and 0.46 mmoL/L,respectively. Under the optimal condition of culture medium, the total oil content and oil yield of Desmodesmus sp. XJ842 reached 67.73% and 29.27 rag/（ L · d）, respectively. After optimization, the total oil content and oil yield respectively increased by 2.08 times and 2.61 times as many as those before optimization. Meanwhile,the contents of saturated fatty acid and monounsaturated fatty acid in Desmodesmus sp. XJ842 oil accounted for 88.41% of the total fatty acid, and the content of linolenic acid was less than 12%, which complied the European Biodiesel Standards （ EN14214 ） and had potential in biodiesel production.
出处
《中国油脂》
CAS
CSCD
北大核心
2016年第1期45-50,共6页
China Oils and Fats
基金
中国博士后科学基金(2013M531370
2014T70532)
关键词
沙漠微藻
链带藻
响应面优化
培养基组成
生物柴油
desert microalgae
Desmodesmus sp.
response surface optimization
cuhure medium composition
biodiesel
