摘要
用不同尿素浓度的叉培养基对三角褐指藻(Phaeodactylum tricornutum)进行培养,研究对该藻的生长和脂肪酸组成的影响。结果表明,尿素的添加明显提高三角褐指藻的细胞密度,生长随尿素浓度增加(0～8.0mmol/L)而增加。二十碳五烯酸(EPA)和多不饱和脂肪酸(PUFAs)含量随尿素浓度增加先上升后下降,在2.1mmol/L时EPA的含量最高达到19.1%。综合生物量与EPA和PUFAs产量,叉培养基中以尿素浓度为2.1mmol/L为宜,此时的细胞密度较高,且有利于EPA和PUFAs的富集。
Phaeodactylum tricornutum was cultured in medium with different urea concentration to investigate its growth and fatty aeid composition. The results showed that adding of urea in medium could improve the cell intensity of P. tricornutum markedly. The growth increased with urea concentration rising from 0 to 8.0 mmol/L. The content of eicosapentaenoic acid （EPA） and polyunsaturated fatty acids （PUFAs） increased at first and then dropped with a maximum EPA content （19.1%） at 2.1 mmol/L of urea. The cell intensity was relatively high when 2.1 mmol/L was set as the optimal urea concentration. Considering biomass of algae and production of EPA and PUFAs, this urea concentration is also suitable to get higher production of both.
出处
《畜牧与饲料科学》
2008年第4期24-26,共3页
Animal Husbandry and Feed Science
