摘要
通过单因素实验确定pH、温度、盐度三个因素下溶藻弧菌的最优培养条件。根据Box-Behnken中心组合实验原理进行响应面分析,以菌液的OD600为响应值,确定影响溶藻弧菌培养的各因素水平。结果表明,最优培养条件为pH7.62、温度34.55℃、盐度3.22%;菌体生长过程中pH和温度、温度和盐度对V.a ATCC17749生长的交互作用显著。
According to singe-factor experiments,three levels factors including the pH,temperature and salinity were selected to evaluate the optimum culture condition of Vibrio alginolyticus. Based on Box-Behnken central composite design principle of the Response Surface Methodology（RSM）,the OD600 was chosen as response value to determine the range of factors and levels. With the results ,the optimized conditions of cultivation were as follows：pH7.62,temperature 34.55℃ and salinity 3.22% ,respectively. During the germination significant interactions between pH and temperature,as well as temperature and salinity were found,
出处
《食品工业科技》
CAS
CSCD
北大核心
2012年第16期221-223,245,共4页
Science and Technology of Food Industry
基金
上海市科委工程中心建设(11DZ2280300)
上海市教育委员会重点学科建设项目资助(J50704)
关键词
溶藻弧菌
Box-Behnken
响应面
培养条件
Vibrio alginolyticus
Box-Behnken
response surface methodology（RSM）
culture condition
