摘要
目的制备Integrinαvβ3靶向超声微泡,对其一般理化性质及体外寻靶能力进行检测。方法采用巯基-马来酰亚胺连接法制备携FITC标记iRGD肽的Integrinαvβ3靶向微泡(MBIntegrinαvβ3),并制备空白微泡(MBcontrol)。检测两组微泡的一般理化性质,在荧光显微镜下观察两组微泡荧光显像,流式细胞仪检测两组微泡FITC荧光含量。于光学显微镜及荧光显微镜下观察两组微泡与小鼠脑微血管内皮细胞(bEnd.3)的黏附情况。结果 1MBIntegrinαvβ3粒径为(0.84±0.26)μm,与MBcontrol粒径(0.79±0.19)μm差异无统计学意义(P>0.05);2荧光显微镜下观察,MBcontrol组微泡无荧光显示,MBIntegrinαvβ3组微泡表面显示绿色荧光;3流式细胞仪测得MBIntegrinαvβ3组微泡FITC荧光含量为98.4%,MBcontrol组微泡FITC荧光含量为2.5%;4体外寻靶实验显示,MBIntegrinαvβ3组微泡能聚集结合于bEnd.3细胞表面。结论成功制备了携iRGD肽的Integrinαvβ3靶向微泡,其具有良好的体外寻靶能力。
Objective To prepare Integrin αvβ3targeted microbubbles, test physical-chemical properties and targeting study in vitro. Methods Integrin avl33 targeted microbubbles （MBIntegrinαvβ3） with FITC labled iRGD peptide were prapared by shmaleic imide connection method. Then control microbubbles （MB 1） was prapared. Physical and chemical properties were test, fluorescent were displayed under fluorescent microscope and the contents of FITC fluorescence by flow cytometry technique of 2 groups of microbubbles were detected. The affinity of MBIntogrinαvβ3 and MB 1for bEnd. 3 ceils was investigated with light and fluorescent microscope. Results ①The particle size of MBintogrinαvβ3 was （0.84±0.26）μm, and had no statistically significant difference compared with MB control（P〈0.05）. ②Under fluorescent microscope, MB l displayed without fluorescent, while MBIntegrinαvβ3 showed clear green light. ③ The contents of FITC of MBIntegriαvβ3 was 98.4%, however, the contents of FITC of MB control was 2.5%. ④It showed that MBIntegrinαvβ3 adhered to bend. 3 ceils in vitro experiment. Conclusion Integrinαvβ3 targeted microbubbles with iRGD is successfully prepared and proves good targeting ability in vitro.
出处
《中国医学影像技术》
CSCD
北大核心
2016年第2期175-178,共4页
Chinese Journal of Medical Imaging Technology
基金
广东省自然科学基金项目(2014A030313760)
深圳市科技创新委员会重大项目(JCYJ20130402092657773)
关键词
整合素ΑVΒ3
造影剂
超声检查
靶向
微泡
Integrin alphaVbeta3
Contrast media
Ultrasonography
Targeted
Microbubbles
