摘要
目的:对比研究18α-甘草酸,18β-甘草酸及二者联合用药对小鼠急性肝损伤的保护作用,并筛选二者联合用药的最佳配伍比例。方法:小鼠随机被分为正常组,模型组,18α-甘草酸,18β-甘草酸低、中、高剂量组(15,30,60 mg·kg^(-1)),联合用药给药剂量均为60 mg·kg^(-1),联合用药1(18α-甘草酸-18β-甘草酸1∶3)组、联合用药2(18α-甘草酸-18β-甘草酸2∶3)组、联合用药3(18α-甘草酸∶18β-甘草酸1∶1)组。在造模前各治疗组ip给药7 d,1次/d,其他各组ip生理盐水;末次给药1 h后,除正常组ip生理盐水外,其他各组均ip 0.1%四氯化碳(CCl_4)花生油(20 m L·kg^(-1))建立CCl_4诱导小鼠急性肝损伤模型,观察18α-,18β-甘草酸高、中、低剂量(15,30,60 mg·kg^(-1))组,联合用药3个不同配伍比例组对急性肝损伤小鼠血清中和肝组织中丙氨酸转氨酶(ALT),天冬氨酸转氨酶(AST)水平的影响,并检测肝组织匀浆中超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量,同时筛选二者联合用药的最佳配伍比例。结果:与正常组比较,模型组血清AST,肝组织AST,ALT,MDA水平明显升高,SOD活力显著降低(P<0.01),提示造模成功。与模型组比较,18α-甘草酸组,18β-甘草酸组均可降低小鼠血清中AST,肝组织AST,ALT活力,MDA含量,升高肝组织中SOD活力;比较二者对急性肝损伤小鼠的保护作用,结果 18β-甘草酸组对肝组织中AST,ALT水平影响优于18α-甘草酸组,其他指标无显著性差异;二者联合用药的最佳配伍比例为2∶3,其对肝组织中AST活力及MDA含量的影响均优于单用18α-或18β-甘草酸治疗组。结论:18α-与18β-甘草酸可保护急性肝损伤小鼠,后者保护作用比前者强,二者配伍的最佳比例是2∶3,其对AST,MDA水平影响优于单用18α-或18β-甘草酸。
Objective： To compare the protective effect of 18α-and 18β-glycyrrhizic acid on acute liver injury in mice,and screen out their optimal combination proportion. Method： The mice were randomly divided into normal group,model group,high dose group（60 mg·kg^-1）,middle dose group（30 mg·kg^-1）,low dose group（15 mg·kg^-1） of 18α- and 18β-glycyrrhizic acid. With the drug dose of combined medication groups of60 mg·kg^-1,combined medication 1 groups（18α-glycyrrhizic acid-18β-glycyrrhizic acid 1 ∶ 3）,combined medication 2 groups（18α-glycyrrhizic acid-18β-glycyrrhizic acid 2 ∶ 3）,combined medication 3 groups（18α-glycyrrhizic acid-18β-glycyrrhizic acid 1 ∶ 1）. Before the modeling,the drugs were injected once everyday for7 days,the same volume of saline was given to normal group and model group. At 1 h after the last administration,the treated groups and model group were ip given 0. 1% CCl4 peanut oil（20 m L·kg^-1） to establish mouse model of acute liver injury induced by CCl4. The samples of blood and liver from high does group,middle dose group,low dose group and three combination proportion groups of 18α- and 18β-glycyrrhizic acid were analyzed,and level of alanine aminotransferase（ALT）, aspartate aminotransferase（AST）, superoxide dismutase（SOD） and malondialdehyde（MDA） were measured. Result： Compared with the normal group,the serum levels of AST,liver homogenate levels of AST,ALT,MDA in model group were remarkably higher（P〈 0.01）,levels of SOD was remarkably lower. Compared with the model group,18α-and 18β-glycyrrhizic acid remarkably reduced the serum levels of AST,reduced liver homogenate levels of AST,ALT and MDA,and increased liver homogenate content of SOD. 18β-glycyrrhizic acid had a better effect on liver homogenate levels of AST, ALT than 18α-glycyrrhizic acid,and showed no significant difference in other indexes compared with 18β-glycyrrhizic acid. The optimal combination proportion of 18α-and 18β-glycyrrhizicacid is 2 ∶ 3,with
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2016年第2期103-106,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家科技部科研行业专项(201407002)
辽宁省科技厅自然科学基金项目(2014010052-301)
关键词
18Α-甘草酸
18Β-甘草酸
急性肝损伤
保护作用
配伍比例
18α-glycyrrhizic acid
18β-glycyrrhizic acid
acute liver injury
protective effects
combination proportion
