摘要
目的研究甘草提取物及18α-甘草酸(18α-GL)、18β-甘草酸(18β-GL)、18α-甘草次酸(18α-GA)、18β-甘草次酸(18β-GA)对HepG2细胞上UGT1A蛋白表达和UGT1A mRNA作用的影响。方法建立HepG2细胞模型,采用Western blot分析HepG2细胞中二相代谢酶UGT1A的蛋白表达,采用Q-PCR从转录水平检测UGT1A mRNA。结果 Western blot实验结果表明18α-GL、18β-GL对UGT1A的蛋白表达有增加作用但差异无统计学意义;18α-GA和18β-GA使UGT1A的蛋白表达量增加且具差异有统计学意义(P<0.05)。Q-PCR实验结果表明18α-GL、18β-GL使UGT1A的mRNA表达有上调作用;18α-GA对UGT1A的mRNA表达有上调作用,但差异没有统计学意义,而18β-GA使UGT1A的mRNA表达上调,且差异有统计学意义(P<0.05)。结论甘草提取物、18α-GL、18β-GL、18α-GA和18β-GA对UGT1A蛋白表达和mRNA影响均表现为诱导作用,且18β-GA对UGT1A的诱导作用差异有统计学意义。
Objective To determine the effect of licorice extract, 18α-glycyrrhizic acid（18α-GL）, 18β-glycyrrhizic acid（18β-GL）, 18α-glycyrrhetinic acid（18α-GA） and 18β-glycyrrhetinic acid（18β-GA） on protein expression and the effect of mRNA UGT1A on the expression of UGT1A in HepG2 cells. Methods HepG2 cell model was established, and the protein expression of UGT1A protein was analyzed by Western blot. The real-time quantitative PCR was used to detect the m RNA UGT1 A from the transcription level. Results Western blot showed that 18α-GL and 18β-GL increased UGT1 A protein expression but not significantly; the UGT1A protein expression increase by 18α-GL and 18β-GA had statistical significance（P 〈 0.05）. The Q-PCR experimenta showed that 18α-GL and 18β-GL upregulated UGT1 A mRNA expression; the expression of 18α-GA m RNA was upregulated, but no significantly. While 18β-GA upregulated the expression of UGT1 A mRNA significantly（P 〈 0.05）. Conclusion Licorice extract, namely 18α-GL, 18β-GL, 18α-GA and 18β-GA can induce the expression of UGT1A protein and mRNA, and the induction of 18β-GA on UGT1A is significant.
出处
《中南药学》
CAS
2016年第6期611-615,共5页
Central South Pharmacy
基金
国家自然科学基金资助项目(No.81360665)
广西自然科学基金面上基金资助项目(No.2015GXNSFAA 139114)
广西自然科学基金青年基金资助项目(No.2013GXNSFBA019 174)
广西卫生厅中医药攻关课题(No.GZGG13-08)
广西医药卫生自筹经费计划课题(No.Z2014318)
