摘要
目的通过分析不同浓度盐胁迫下甘草酸积累量和总糖、粗蛋白、粗纤维、粗脂肪及灰分量的变化以及它们的相关性,研究盐胁迫对甘草酸积累的影响。方法采用不同质量浓度(3、6、9 mg/mL)NaCl溶液处理一年生盆栽甘草,分别于35、70、105 d取样,测定甘草酸量,总糖、粗蛋白、粗纤维、粗脂肪及灰分5种组分的量,分析盐胁迫对各组分比例关系的影响及甘草酸量与各组分量的相关性。结果盐胁迫70 d时,6、9 mg/mL盐溶液处理组的甘草酸量显著高于对照(CK);6、9 mg/mL处理组的粗蛋白显著高于CK,而9 mg/mL处理组的总糖显著低于CK。盐胁迫105 d时,9 mg/mL处理组的粗脂肪显著高于CK;盐胁迫70 d和105 d时,9 mg/mL处理组的粗脂肪比例显著高于CK,但总糖比例明显低于CK;盐胁迫70 d和105 d时,甘草酸量与粗脂肪、灰分量呈正相关,与总糖量呈负相关。结论甘草酸的积累与粗蛋白、总糖量、粗脂肪、灰分量的分配密切相关,适当的盐胁迫可以刺激甘草内的糖代谢,加速物质的分解,促进甘草的次生代谢,使甘草酸形成并积累。
Objective To investigate the effect of salt stress at different concentrations on the accumulative contents of glycyrrhizic acid and five components and their correlations. Methods Annual Glycyrrhiza uralensis cultivated in the plastic flowerpot was treated with salt at different concentrations （3, 6, and 9 mg/mL）. The contents of glycyrrhizic acid, total sugar, crude protein, crude fiber, crude fat and ash were determined after 35, 70, and 105 d treatment. The proportional relationships of five components and the correlation of glycyrrhizinic acid and five components were analyzed. Results On 70 d after the treatment of 6 and 9 mg/mL salt solution, the glycyrrhizic acid content was extremely higher than that in control group. Crude protein in the treatment groups of 6 and 9 mg/mL salt solution increased significantly compared with that in control group. While total sugar content decreased significantly compared with that in control group. On 105 d, crude fat content in the treatment group of 9 mg/mL salt solution was extremely higher than that in control group. And on 70 and 105 d, the crude fat proportion in the treatment group of 9 mg/mL salt solution was extremely higher than that in control group, while the total sugar proportion was extremely lower than that in control group. The correlation analysis showed that glycyrrhizic acid content had a positive correlation with the content of crude fat and ash, while had a negative correlation with total sugar on 70 and 105 d. Conclusion The accumulation of glycyrrhizic acid is closely related to proportion of crude protein, total sugar, crude fat and ash content. The proper salt stress could stimulate sugar metabolisms and accelerate the decomposition of the substance. All of these could promote the secondary metabolism and make the formation and accumulation of glycyrrhizic acid.
出处
《中草药》
CAS
CSCD
北大核心
2011年第11期2312-2316,共5页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(30572328)
关键词
甘草
盐胁迫
甘草酸
总糖
粗蛋白
粗纤维
粗脂肪
灰分
Glycyrrhiza uralensis Fisch.
salt stress
glycyrrhizic acid
total sugar
crude protein
crude fiber
crude fat
ash
