摘要
目的 探讨丰富环境对快速老化小鼠SAMP8轻度认知功能障碍（Mild cognitive impairment,MCI）阶段认知状况的影响及其可能的调节机制.方法 采用健康雄性MCI阶段（5月龄）SAMP8小鼠20只,随机均分为丰富环境（enriched environment,EE）组（简称P8 EE组）和标准环境（standard environment,SE）组（简称P8 SE组）两组,分别在相应环境下饲养60 d,采用健康雄性同月龄SAMR1小鼠10只,作为正常的对照组（简称R1 SE组）,在SE下饲养60 d.通过Morris水迷宫实验检测干预前、后各组小鼠的认知状况;免疫组化染色和图像分析系统检测干预后各组小鼠海马CA1区β-淀粉样蛋白（amyloid protein beta,Aβ）的沉积和胶质纤维酸性蛋白（glial fibrillary acidic protein,GFAP）的表达.结果 水迷宫实验中,各组小鼠干预前、后平均潜伏期和跨越平台次数差异都有统计学意义（均P＜0.05）,干预后P8 EE组小鼠4天的平均潜伏期[分别为（81.22±11.82）s,（76.59± 13.36）s,（70.74±8.73）s,（66.40±7.79）s]明显短于干预后P8SE组小鼠（P＜0.01）,干预后P8 EE组小鼠跨越平台次数[（9.31±1.33）分]明显多于干预后P8 SE组小鼠（P＜0.01）,干预后P8 EE组小鼠与干预后R1SE组小鼠相比,上述指标之间的差异都有统计学意义（均P＜0.01）;免疫组化实验中,干预后P8 EE组小鼠Aβ和GFAP平均吸光度[分别为（0.26±0.02）,（0.26±0.03）]明显少于干预后P8 SE组小鼠（P＜0.01）,干预后P8 EE组小鼠与干预后R1 SE组小鼠之间两个指标的差异也有统计学意义（均P＜0.01）.结论 丰富环境干预可减轻SAMP8小鼠MCI期的认知缺损,可能与抑制海马区Aβ沉积,降低GFAP的表达,抑制星形胶质细胞的活化有关.
Objective To investigate the effects of enriched environment on cognitive situation in senescence accelerated mouse prone/8 （SAMP8） with mild cognitive impairment （MCI） and the possible regulatory mechanisms.Methods A total of 20 healthy male SAMP8 mice in MCI （5-month-old） were randomly divided into enriched environment group （short for P8 EE group） and standard environment group （short for P8 SE group）with 10 in each group.Two groups of SAMP8 mice were subjected to the two different environments for 60 days.10 5-month-old healthy male SAMR1 mice were selected as normal control group （short for R1 SE group） and subjected to the standard environment for 60 days.The cognitive situation of mice in each group before and after intervention was examined by the Morris water maze （MWM） test.The deposition of amyloid protein beta（A3） and expression of glial fibrillary acidic protein（GFAP） in hippocampal CA1 of mice in each group after intervention were determined by immunohistochemical staining and image analysis system.Results In the MWM test,there were statistical differences of latency and times of crossing the platform before and after intervention in each group（all P 〈0.05）.The average latency （（81.22±11.82） s,（76.59± 13.36） s,（70.74±8.73） s,（66.40±7.79） s） of P8 EE group after intervention were significantly shorter than that of P8 SE group on four consecutive days （P〈0.01）,while the times of crossing platform in P8 EE group after intervention （9.31±1.33） were more than that in P8 SE group after intervention （P〈0.01）.There were significant differences of escape latency and crossing times between P8 EE group and R1 SE group after intervention （all P〈0.01）.By immunohistochemical staining,the average absorbance of Aβ and GFAP（（0.26±0.02）,（0.26±0.03）） in P8 EE group after intervention were significantly less than that in P8 SE group after intervention （P〈0.01）.There were significant differences of A3 and GFAP l
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2015年第11期974-977,共4页
Chinese Journal of Behavioral Medicine and Brain Science
基金
山西省卫生计生委科研项目（2015158）
山西省高等学校创新创业训练项目（2015303）
关键词
丰富环境
学习记忆
Β-淀粉样蛋白
胶质纤维酸性蛋白
轻度认知功能障碍
Enriched environment
Learning and memory
Amyloid protein beta
Glial fibrillary acidic protein
Mild cognitive impairment
