摘要
目的探讨麦角甾醇对S180荷瘤小鼠抑瘤作用及机制。方法建立S180荷瘤小鼠模型,将50只荷瘤小鼠随机分为模型组、环磷酰胺组,高、中、低剂量麦角甾醇组,每组10只,雌雄各半;连续给药21 d,计算抑瘤率;苏木素-伊红染色,观察肿瘤组织生长情况;测定肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSHPx)活力和丙二醛(MDA)含量;Western blot法检测肿瘤组织中survivin蛋白表达。结果与模型组比较,环磷酰胺组、高、中、低剂量麦角甾醇组小鼠抑瘤率分别为53.96%、41.64%、29.90%、19.52%,差异均有统计学意义(P<0.05);各麦角甾醇组小鼠肿瘤组织内均出现不同程度微血管减少和多种凋亡形态学改变;与模型组比较,高、中剂量麦角甾醇组小鼠肝脏中SOD、GSH-Px活力[分别为(69.19±12.18)、(66.30±8.80)与(6 982.69±1 141.96)、(6414.43±932.96)U/mg prot]明显升高(P<0.05),MDA水平[分别为(24.59±6.87)、(31.43±7.05)nmol/mg prot]明显降低(P<0.05);与模型组比较,高、中剂量麦角甾醇组小鼠肿瘤组织中survivin蛋白表达水平下调(P<0.05)。结论麦角甾醇对S180荷瘤小鼠具有抑瘤作用,其机制可能与提高小鼠肝脏抗氧化能力和降低肿瘤组织中survivin蛋白表达水平有关。
Objective To investigate antitumor effect of ergosterol and its mechanism in S180 tumor-bearing mice. Methods S180 tumor-bearing model was established in 50 mice（ half male and female）and the mice were randomly divided into five groups （ 10 in each group） ： model group, cytoxan （CTX） group, high-, medium-, and low-dose ergosterol groups. The tumor inhibition rate in the mice of different groups was calculated 21 days after continuous treatment. Hematoxylin-eosin staining was used to observe proliferation of the tumors. The levels of superoxide dismutase （ SOD）, glutathion peroxidase（GSH-Px） and malondialdehyde （MDA） in liver tissues were evaluated. Western blot was used to determine the expression of survivin protein in tumors. Results Compared with the model group, the inhibitory rates of CTX group, high-, medium-, and low-dose ergosterol group were 53.96% ,41.64% ,29.90%, and 19. 52%, with a statistically significant difference（ P 〈 0. 05 ）. Different extents of decreased capillaries and a variety of morphological apoptosis changes in tumor tissues were observed in the mice of ergosterol groups. Compared with the model group, the contents of SOD （69. 19 ± 12. 18,66. 30 ± 8.80 U/mg prot） and GSH-Px （ 6 982. 69 ±1 141.96,6 414.43 ± 932.96 U/mg prot） in the liver tissues of high- and medium-dose ergosterol groups were significantly increased（ all P 〈 0. 05 ） , while the content of MDA （ 24. 59± 6. 87,31.43 ± 7.05 nmol/mg prot） was significantly decreased （ P 〈 0. 05 ）. Compared with the model group, the expression level of survivin protein was significantly decreased（P 〈 0. 05 ）in tumors of the mice with high- and me- dium-dose ergosterol treatment. Conclusion Ergosterol has antitumor effect in S180 tumor-bearing mice and the mechanism of the effect may relate to the upregulation of antioxidant capacity in liver and downregulation of survivin protein expression in tumor.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2015年第12期1606-1608,共3页
Chinese Journal of Public Health
基金
公益性行业(农业)科研专项经费资助(201303080)
