摘要
应用三波长光度法测定了姬松茸菌丝体中麦角甾醇的含量。先采用薄层色谱将待测正庚烷萃取液中的麦角甾醇和其它杂质分离,然后分别进行紫外扫描,确定三波长为272,282和292nm,计算出ΔA,因ΔA仅与姬松茸菌丝体中麦角甾醇含量相关而不受杂质影响,从而有效地消除了杂质紫外吸收的干扰。回归方程为：ΔA=0.00403C＋0.001048,相关系数r=0.9996,麦角甾醇的浓度在0-50mg/L范围内,ΔA与浓度C之间呈良好的线性关系。本法的相对标准偏差RSD为1.2%（n=5）,回收率为97.5%-102.5%。方法的精密度与准确度令人满意,所得结果与HPLC法相近,而且操作简便易行。
The ergosterol content in mycelia of Agaricus brasiliensis was determined by triple-wavelength spectrophotometry. Ergosterol was separated from other impurities using TLC method. According to the UV scan curve of ergosterol and impurities, the triple wavelengths were identified as 272 nm, 282 nm and 292 nm. The value of ΔA is only relevant to the ergosterol concentration. Therefore it can eliminate the absorbance error caused by interfering components. The regression equation of concentration vs ΔA was obtained ： ΔA = 0. 00403C ＋0. 001048 with a correlation coefficient of r = 0. 9996, and a linear relationship was obtained in the ergosterol concentrations of 0 - 50 mg/L with ΔA. The relative standard deviation （RSD） of precision test was 1.2% （n =5 ） and the recovery of standard addition is 97.5% -102.5% . The result of the method is close to that of HPLC method. The method is easier and cheaper than HPLC method, and more accurate than single-wavelength spectrophotometry.
出处
《分析化学》
SCIE
EI
CAS
CSCD
北大核心
2007年第4期586-588,共3页
Chinese Journal of Analytical Chemistry
关键词
姬松茸
麦角甾醇
三波长光度法
含量测定
Agaricus brasiliensis, ergosterol, triple-wavelength spectrophotometry
