摘要
为了获得适用于发酵生产Nisin的海藻酸钠(A)-壳聚糖(C)-海藻酸钠(A)乳链球菌6032的液芯微囊,以其发酵液吸光度值OD600、Nisin的效价为综合指标,探讨了影响ACA乳链球菌6032液芯微囊发酵性能的制备工艺条件.在单因素实验的基础上采用Box-Behnken模型响应面设计,建立了乳酸菌ACA液芯微囊制备的三元二次回归模型,并确定了其制备因素的最佳水平值为壳聚糖溶解时间2h、搅拌速度236r/min、制备温度33.7℃、滴加速度4.9mL/min.将此条件下制得的乳酸菌ACA液芯微囊发酵生产Nisin,吸光度值OD600为0.181,Nisin效价为5 242IU/mL,该结果与模型预测值拟合良好,说明响应面分析法优化该实验是有效的.
In order to obtain the ACA liquid core microcapsules of lactococcus lactis 6032 for Nisin fermentation ,absorbance value of OD600 and titer unit of Nisin in the broth as compre‐hensive indicators ,the liquid core microcapsules preparation conditions affect on fermentation of L actobacillus was discussed .Based on one‐factor experimental design ,the quadratic model was founded by the Box‐Behnken response surface model .The optimal value of each prepara‐tion condition were chitosan dissolution time 2 h ,stirring speed 236 r/min ,temperature 33 .7℃ ,dropping rate 4 .9 mL/min .Under these conditions ,the absorbance value of OD600 was 0 .181 and the titer unit of Nisin was 5 242 IU/mL ,which is consistent with the predicted value .These showed that it is effective for optimizing preparation conditions by response sur‐face methodology .
出处
《陕西科技大学学报（自然科学版）》
2015年第6期132-137,共6页
Journal of Shaanxi University of Science & Technology
基金
国家自然科学基金青年项目(31301059)
陕西省教育厅自然科学专项科研计划项目(14JK1089)
关键词
制备工艺条件
发酵性能
ACA乳酸菌液芯微囊
preparation conditions
fermentation
ACA liquid core microcapsules of L actoba-cillus
