摘要
目的观察热疗联合阿霉素对人多发性骨髓瘤细胞株ARH-77细胞内的药物浓度变化及凋亡的影响。方法 MTT法确定阿霉素的工作浓度,以该浓度进行化疗或与热疗的联合,选择温度40℃、41℃及42℃,体外作用于ARH-77细胞。作用前及48 h采用台盼蓝拒染法检测肿瘤细胞的存活率;MTT法检测肿瘤细胞增殖的抑制作用;流式细胞仪检测肿瘤细胞的凋亡及细胞内药物浓度的变化。观察热疗联合阿霉素的抗肿瘤效果。结果作用48h IC50的药物浓度作为实验的工作浓度。热化疗组对ARH-77细胞有明显的抑制作用(P<0.01),随着温度的增高而增强;热化疗组细胞内的药物浓度明显增加(P<0.01)。结论热疗联合阿霉素能增加ARH-77细胞内的药物浓度,提高肿瘤细胞的凋亡率。
Objective：To observe the effect of thermotherapy on the intracellular adriamycin concentration and apoptosis of ARH-77 cells in vitro. Methods：The working concentration of adriamycin against ARH-77 was determined by MTT as-say. ARH-77 cells were subjected to thermotherapy（ at 40℃,41℃,42℃）and chemotherapy with adriamycin alone or in conjunction,and the cell survival rates were determined at 48h after the treatment. The cell growth inhibition effect of the treatment was evaluated with MTT assay,and the apoptosis rates of ARH-77 and alteration of intracellular adriamycin con-centration were determined by flow cytometric analyses. Results：The IC50 of adriamycin was defined as the working con-centration in the experiment. The thermotherapy at 40,41 and 42℃ for 60 min in conjunction with chemotherapy signifi-cantly inhibited the growth of ARH-77 cells（P﹤0. 01）. The results of flow cytometric analyses showed that thermotherapy and adriamycin chemotherapy,used either alone or in conjunction,obviously increased the apoptosis rates of ARH-77 cells （ P﹤0. 01）and thermotherapy remarkably increased the intracellular concentration of adriamycin. Conclusion：Adriamycin chemotherapy combined thermotherapy for 60min can increase the intracellular concentration of adriamycin and the apopto-sis rates of ARH-77 cells.
出处
《泰山医学院学报》
CAS
2015年第9期967-970,共4页
Journal of Taishan Medical College
基金
CSCO-先声抗肿瘤血管靶向治疗科研基金(Y-S2014-016
省级)
关键词
热化疗
阿霉素
ARH-77
增敏作用
凋亡
thermochemotherapy
adriamycin
ARH-77 cells
sensitization
apoptosis
