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蟾蜍灵抑制人多发性骨髓瘤细胞生长的实验研究

The effects of Bufalin in inhibiting proliferation on human myeloma cell line U266
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摘要 目的研究蟾蜍灵对人多发性骨髓瘤细胞U266的作用。方法台盼蓝拒染法观察蟾蜍灵对细胞增殖的抑制作用。结果 12.5~100nmol/L蟾蜍灵处理U266细胞12~72 h后,蟾蜍灵以时间、剂量依赖方式抑制U266细胞增殖。12.5~100nmol/L/L蟾蜍灵于24h开始明显抑制U266细胞生长(P<0.05)。结论蟾蜍灵以时间、剂量依赖方式抑制U266细胞生长。 【Objective】 To investigate the effects of bufalin on inhibiting proliferation in human myeloma cell line U266.【Methods】 The proliferation of U266 cells were determined by trypan blue dye exclusion.【Results】 Cell viability was significantly decreased by treatment with 12.5nmol/L bufalin for 24 h(P 0.01).The inhibition of bufalin on U266 expressed does-and time-dependent manner.【Conclusion】 Bufalin could significantly suppress the growth of U266 cells in a does-and time-dependent manner.
出处 《中国医学工程》 2012年第2期5-5,8,共2页 China Medical Engineering
基金 国家自然科学基金资助项目(81101487) 教育部归国留学回国人员科研启动基金项目(教外司留2011508) 教育部高等学校博士学科点专项科研基金(20102104120014) 辽宁省教育厅科研项目基金(L2010640)
关键词 骨髓瘤细胞 蟾蜍灵 细胞生长 myeloma cells bufalin proliferation
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