摘要
【目的】对基地和市售岗梅商品药材进行质量调查和评价。【方法】参照2010年版《中华人民共和国药典》附录ⅨH、ⅩA方法检测19批商品药材的醇溶性浸出物,采用《广东省中药材标准》中岗梅项下方法对药材进行显微鉴别,并优化薄层鉴别,建立岗梅指纹图谱。指纹图谱采用Waters XBridge TM C18色谱柱（250 mm×4.6 mm,5μm）,以乙腈—体积分数0.2%磷酸梯度洗脱,流速1.0 m L/min,检测波长220 nm。【结果】样品性状、显微及薄层鉴别结果显示：收集的样品为正品,但有根茎混用现象;高效液相色谱（HPLC）指纹图谱分析结果通过相似度分析软件确定了7个共有色谱峰,检测的15批基地药材相似度均达到0.9以上,19批商品岗梅药材中有11批相似度达到0.9以上;样品醇溶性浸出物范围为64.55~186.18mg/g。【结论】收集的商品药材均为正品,但根茎混用以及生长年限不够等现象导致质量参差不齐,基地药材质量较好;本研究建立的质量评价方法可为岗梅根药材的质量评价与质量控制提供参考依据。
Objective To evaluate the quality of Radix et Caulis Ilicis Asprellae from Pingyuan planting base and Chinese herbal medicine market. Methods The water-and alcohol-soluble extracts from 19 batches of Radix et Caulis Ilicis Asprellae medicinal materials were detected according to Appendix Ⅸ H, Ⅹ A of the Chinese Pharmacopoeia（ 2010 edition）. And the quality of the medicinal materials was evaluated by microscopic identification technology according to the method for Radix et Caulis Ilicis Asprellae recorded in Guangdong Provincial Chinese Medicine Standard, and then thin layer chromatography（ TLC） was optimized to establish the high performance liquid chromatography（HPLC） fingerprint. The HPLC was performed on Waters XBridgeTM C18column（250 mm × 4.6 mm, 5 μm） with acetonitrile（A）-0.2%（v/v） phosphorus acid（B） as the mobile phase by gradient elution, flow rate was 1.0 m L / min, and detection wavelength was 220 nm. Results The results of sample characters, TLC and microscopic identification showed that the samples of Radix et Caulis Ilicis Asprellae in Chinese herbal medicine markets were certified products, but stems and roots were blended.Seven common peaks were showed by HPLC and confirmed by similarity analytical software. The similarity of 15 batches of planting base samples was all above 0.9. Of 19 batches of the commercial samples, the similarity of11 batches was above 0.9. The alcohol-soluble extract contents were in the range of 64.55 mg / g to 186.18 mg / g.Conclusion The medicinal materials of Radix et Caulis Ilicis Asprellae from Chinese herbal medicine market are certified products, but the qualities vary greatly for the blending of stems and roots and inadequate growth years.The quality of materials from planting base is better. The established method is helpful for the quality evaluation and control of Radix et Caulis Ilicis Asprellae.
出处
《广州中医药大学学报》
CAS
2015年第6期1082-1086,1147-1149,共8页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省科技计划项目(编号:2012A030100006)
广东省协同创新中心创新科研团队建设项目(编号:A1-AFD01514A04)
关键词
岗梅/化学
质量评价
色谱法
高压液相
色谱法
薄层扫描
Radix et Caulis Ilicis Asprellae / chemistry
Quality evaluation
Chromatography
high performance liquid
Chromatography
thin layer
