摘要
目的 了解北京市房山区腹泻病人中轮状病毒和人杯状病毒的感染情况.方法 2011年4月-2013年3月收集北京市两家医院肠道门诊腹泻患者便标本243份,采用ELISA检测轮状病毒抗原,采用一步法RT-PCR对人杯状病毒核酸进行检测.结果 60份样本病毒检测阳性,轮状病毒检出率为10.3％（25/243）,人杯状病毒检出率为14.4％（35/243）.60岁以上患者轮状病毒阳性检出率为12.1％（4/33）,高于其他年龄组.12月份到次年3月份为轮状病毒腹泻的高发季节,人杯状病毒腹泻的季节性特征不明显.结论 轮状病毒和人杯状病毒是北京市房山区肠道门诊腹泻患者的重要病原体.
Objective To understand the epidemiological characteristics of rotavirus and human calicivirus （HuCV） infection among patients with diarrhea in Fangshan district.Methods A total of 243 stool samples were collected from diarrhea patients at 2 sentinel hospitals.Rotavirus antigen was detected by ELISA, and the nncleic acid of HuCV was detected by one step reverse transcriptase polymerase chain reaction （RT-PCR）.Results Sixty samples were virus-positive, and the detection rates of rotavirus and HuCV were 10.3% （25/243）, 14.4% （35/243）, respectively.The highest detection rate of rotavirus （12.1%, 4/33） was found among patients over 60 years old.The rotavirus infections were prevalent during DecemberMarch, and the seasonality of HuCV infections was not significant.Conclusions Rotavirus and HuCV were the important causes of diarrhea in Fangshan district, Beijing.
出处
《国际病毒学杂志》
2015年第5期328-331,共4页
International Journal of Virology
