摘要
诺瓦克病毒是引起无菌性急性胃肠炎爆发的重要病原。１９９０年１０月至１９９１年１月从河南省急性腹泻门诊患儿便样中发现了诺瓦克样病毒。经电镜观察，病毒直径约为２８ｎｍ，病毒壳似由有结构的亚单位组成，进一步用诺瓦克病毒特异的寡核苷酸引物做逆转录一聚合酶链式反应（ＲＴ－ＰＣＲ），检定为阳性。由ＰＣＲ产物测得的核苷酸序列与诺瓦克病毒原型株相应序列比较，同源性为７２％。以上结果证实，在我国腹泻病人中有诺瓦克样病毒感染。这对我国病毒性胃肠炎流行的防治研究具有重要意义。
Norwalk virus or Norwalk virus -like agents are important pathogens that causeoutberaks of acute nonbacterial gastroenteritis. Two Norwalk-like virus isolates were identifiedin fecal specimens from acute diarrhea patients in Henan province during Oct.1990-Jan1991 rotavirus season by electron microscopy that shows 28nm diameter with structured capsid.RT-PCR using Norwalk -specific primer pair 51-3,and nucleotidesequencing of PCR products showed 72%homology of these two isolates to that of Norwalkvirus prototype 8FⅡa. Our finding suggests that more attention should be pai to outbreaks of acute gastroenteritis caused by Norwalk-like virus in China.
出处
《病毒学报》
CAS
CSCD
北大核心
1995年第3期215-219,共5页
Chinese Journal of Virology
基金
国家自然科学基金
日本厚生省援助基金
