摘要
为了优化猪苓多糖的微波提取工艺,采用Box-Behnken响应面设计法,研究液料比、p H、微波功率、提取时间、提取次数及其交互作用对多糖提取率的影响。应用Design Expert和响应面分析相结合的方法,模拟得到回归方程的预测模型和可信度,分析得到最佳提取工艺条件为:液料比30∶1(m L/g),p H 6.6,微波功率614 W,提取时间2.5 min,提取次数2次。在此条件下,多糖提取率达到6.75%。利用Box-Behnken响应面设计法优化得到的猪苓多糖提取条件参数,为猪苓多糖工业化生产提供技术支持。
To optimize the microwave-assisted extraction technique of polysaccharide from Polyporus umbellatus,the effects of the liquid to solid ratio,p H,microwave power,extraction time,extraction number and their interaction on extaction were studied by Box-Behnken design. The predictive model and reliability were developed by Design Expert software and response surface analysis（RSM）. The optimal extraction conditions were achieved and listed as follows：liquid-to-solid ratio 30 ∶1 m L/g,p H 6.6,microwave power 614 W,extraction time 2.5 mins,extraction number 3 times. Under these conditions,the extraction yield of polysaccharide was up to6.75%. The extraction conditions achieved by RSM provided technical support for the industrial production of polysaccharide from Polyporus umbellatus.
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2015年第9期986-994,共9页
Journal of Food Science and Biotechnology
基金
陕西省"春笋计划"研究项目(2014-2015)
关键词
猪苓
微波辅助提取
多糖
BOX-BEHNKEN设计
响应面分析
Polyporus umbellatus
microwave-assisted extraction
polysaccharides
Box-Behnken design
response surface analysis
