摘要
利用响应面方法对重组谷胱甘肽硫转移酶表达菌株E coliBL21(DE3)PGEX发酵生产谷胱甘肽硫转移酶(GST)的培养基进行了优化。用Plackett-Burman实验方法研究葡萄糖、酵母膏和MgSO4等20个营养因子对产GST活力的影响,结果表明主要影响因子为葡萄糖、酵母膏和MgSO4。根据实验结果对主要影响因子的浓度范围进行估计,然后用Box-Behnken设计及响应面分析确定主要影响因子的最佳浓度。结果表明当葡萄糖浓度为42.06 g/L,蛋白胨浓度为10g/L,酵母膏浓度为8.47 g/L,NaCl浓度为1 g/L,MgSO4浓度为1.62 g/L时,E coliBL21(DE3)PGEX产GST活力达到633.9 mmol/(L.h),较原始培养基的活力提高了63.75%。
Response surface design was used to optimize the medium components for Glutathione S-transferases (GST) fermentation from E coli BL21(DE3)P^GEX. Firstly, 20 nutrients factors' effects on the fermention such as glucose, yeast extract, MgSO4 etc were evaluated by using Plackett-Burman design. Glucose, yeast extract and MgSO4 played important roles in GST production, while others had minor effect. Accordillg to the result of the research, the scope of the influential factors could be estimated, then a Box-Behnken design was employed to optimize the significant factors and the results were shown in response surface plots. The maximum activity of GST under optimum medium was 633.9 mmol/( L· h) which was 63.75 % higher than that of the original medium. The optimum medium were 42, 06 g/-L glucose, 10 g/-L peptone, 8.47 g/-L YE, 1 g/-L NaC1 and 1.62 g/-L MgSO4.
出处
《武汉理工大学学报》
EI
CAS
CSCD
北大核心
2007年第11期35-39,97,共6页
Journal of Wuhan University of Technology
基金
湖北省自然科学基金(2003ABA126)