摘要
目的探讨4种饮用水对小鼠肝肾功能及各脏器锌铁含量的影响。方法分别测定纯水、自来水、矿泉水和过滤水中锌、铁浓度。将80只清洁级ICR小鼠随机分为4组,每组20只,雌雄各半,分别用纯水(对照组)、自来水、矿泉水和过滤水喂养,90 d后眼球采血,测定小鼠血清谷丙转氨酶(ALT)、总蛋白(TP)、尿素氮(BUN)、尿酸(UA)及肌酐(Cr)含量,随后立即处死小鼠,取肝脏、肾脏、心脏、胰腺及脑测定锌、铁含量,并制作肝肾组织石蜡切片观察其形态学变化。结果过滤水组小鼠血清Cr含量[(36.00±8.83)μmol/L]明显低于纯水组、自来水组及矿泉水组[(44.83±12.64)、(44.56±10.52)、(43.79±10.53)μmol/L],差异有统计学意义(P<0.05);纯水组小鼠肝脏、肾脏锌含量[(33.17±6.18)、(16.69±8.12)μg/g]明显低于过滤水组[(45.31±7.32)、(22.61±6.01)μg/g],差异有统计学意义(P<0.05);小鼠血清Cr与肾脏锌含量呈显著负相关关系(r=-0.300,P<0.05);不同组小鼠血清ALT、TP、BUN、UA及各脏器内铁含量差异均无统计学意义,同时肝肾组织结构未见异常。结论长期饮用过滤水有利于肾小球滤过屏障功能的发挥,其可能与肾锌水平提高有关。
Objective To explore the effect of different drinking water on hepatic and renal function and zinc and iron concentrations of different organs in mice. Methods Zinc and iron concentrations were measured in pure water,tap water,mineral water and filtered tap-water,respectively. 80 ICR mice（ half male and half female） were randomly divided into four groups and fed with pure water（ control group）,tap water,mineral water and filtered tap-water,respectively. After 90 days,blood samples were taken from the eyeballs of mice. The contents of serum ALT,TP,BUN,UA and Cr were analyzed. Then mice were killed and livers,kidneys,hearts,pancreas,brains were removed immediately to determinate zinc and iron concentrations. Simultaneously morphological changes of liver and kidney paraffin section were observed. Results Contents of serum Cr in filtered tap-water group（ 36. 00 ± 8. 83 μmol / L） were lower than those of pure water group（ 44. 83 ±12. 64 μmol / L）,tap water group（ 44. 56 ± 10. 52 μmol / L） and mineral water group（ 43. 79 ± 10. 53 μmol/L）（ P〈0. 05）. The zinc concentrations of livers and kidneys（ 33. 17 ± 6. 18 and 16. 69 ± 8. 12 μg /g） in pure water group was lower than that of filtered tap-water group（ 45. 31 ± 7. 32 and 22. 61 ± 6. 01 μg / g）（ P〈0. 05）. A significant negative correlation（ r =- 0. 300） emerged between the serum Cr and the zinc concentration of kidney in mice（ P〈0. 05）. Conclusion Long-term drinking filtered tap-water is beneficial to the glomerular filtration barrier,and it may be related to the increase of zinc levels in kidney.
出处
《卫生研究》
CAS
CSCD
北大核心
2015年第5期802-805,831,共5页
Journal of Hygiene Research
基金
福建省科技计划重大项目(No.2012Y4004)
关键词
饮用水
肝脏
肾脏
肌酐
锌
铁
drinking water
liver
kidney
creatinine
zinc
iron
