摘要
目的：建立益肾养元颗粒中陈皮的薄层色谱鉴别与橙皮苷含量的测定方法。方法：采用薄层色谱法对陈皮的定性鉴别,采用高效液相色谱法测定橙皮苷的含量;色谱柱为迪马C18柱（150×4.6,5μm）,流动相为乙腈-0.1%磷酸（20∶80）,流速1 m L/min,柱温45℃,检测波长283 nm。结果：薄层色谱鉴别斑点清晰,阴性对照无干扰;橙皮苷进样量为0.114~2.29μg,与峰面积呈良好线性关系（r=0.999 4）,平均回收率为99.67%,平均峰面积（RSD）为2.1%。结论：所建立的方法简便、准确、重复性好,可用于益肾养元颗粒中陈皮的鉴别和橙皮苷的含量测定。
Objective ： To establish the method for the identification of Pericarpium citri reticuLatae in Yishenyangyuan granules and the hesperidin content determination. Methods： Pericarpium cirri reticu- latae were identified by TLC and the content of hesperidin was determined by HPLC. A Diamonsil C18 column （150 mm ×4.6,5μm,5 p,m） was used with a mobile phase consisting of acetonitrile -0.1% phosphoric acid （20： 80） at a flow rate of 1.0 mL/min, the column temperature was 45 ℃ and the de- tection wave length was 283 nm. Result： The bilirubin spot of TLC were clear, and there was no inter- ference in negative control. The sample size of hesperidin was in the range of 0. 114 - 2.29 μg, dem- onstrating a good linear relationship with the peak area （r = 0. 999 4）. The average recovery rate was 99.67% , and RSD was 2.1%. Conclusion： The above established methods were simple and accurate with good reproducible characteristic, which can be used for the identification of Pericarpium citri retic- ulatae and content determination of hesperidin in Yishenyangyuan granules.
出处
《贵阳医学院学报》
CAS
2015年第10期1036-1039,共4页
Journal of Guiyang Medical College
基金
贵州省中药现代化专项项目[黔科合中药字(2013)5062号
黔科合重G字(2013)4001]
贵州省高等学校创新能力提升计划[黔教合协同创新字(2013)04]
关键词
色谱法
薄层
益肾养元颗粒
陈皮
色谱法
液相
橙皮苷
chromatography, thin layer
Yishenyangyuan granules
Pericarpium cirri reticulatae
chromatography,liquid
hesperidin
