摘要
目的:观察丹蒌片对动脉粥样硬化兔血脂、炎症因子及PI3K/AKT信号通路的影响,并探讨丹蒌片抗动脉粥样硬化的机制。方法:雄性日本大耳白兔24只,随机分为正常对照组、模型组、丹蒌片组,每组8只。正常对照组饲喂普通饲料,其余各组高脂饲料喂养制备动脉粥样硬化模型;除高脂饲料喂养外,丹蒌片组(0.5 g·kg-1·d-1)胃饲相应药物。连续给药9周后处死,测各组血脂、血清肿瘤坏死因子(TNF-α)和白介素-6(IL-6)的含量;HE染色观察主动脉病理学改变;Western blot检测主动脉中PI3K和p-AKT的蛋白表达水平。结果:与正常对照组比较,模型组中甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白(LDL)及IL-6、TNF-α的浓度均显著升高(P<0.01),主动脉组织中PI3K、p-AKT的蛋白表达水平明显降低(P<0.01)。与模型组相比,丹蒌片组血脂及IL-6、TNF-α水平明显降低(P<0.05或P<0.01),PI3K、p-AKT的蛋白表达显著升高(P<0.01)。结论:丹蒌片具有降低兔血脂、炎症因子,抗动脉粥样硬化的作用,其作用机制可能与激活PI3K/AKT信号通路相关。
This study was aimed to investigate the effect of Danlou pills on prevent atherosclerosis from hypercholesterolemia rabbit and its relationship with inflammatory factors as well as PI3K/AKT signal pathways. A total of 24 Japanese male white rabbits were randomly divided into the control group（CL）, model group（M） and Danlou group（DL）, with 8 in each group. Normal diet was given to CL rabbits. High-fat diet was given to rabbits in other groups to establish the atherosclerosis model. Danlou pills（0.5 g·kg^-1·d^-1） were also given to DL rabbits. Rabbits were sacrificed after 9-week medication. The contents of blood lipid, TNF-α and IL-6 were detected. HE staining was used in the observation of histological changes in the aorta. Western blot was used to observe PI3 K and p-AKT expression in the aorta. The results showed that compared with CL, the contents of TG, TC, LDL, IL-6 and TNF-α were significantly increased in M（P〈0.01）; PI3 K and p-AKT expression in the aorta were significantly decreased（P〈0.01）. Compared with M, blood lipid, IL-6 and TNF-α were significantly reduced in DL（P〈0.05, or P〈0.01）; PI3 K and p-AKT expression were significantly increased（P〈0.01）. It was concluded that Danlou pills had prevention effects on atherosclerosis through reducing blood lipid and inflammatory factors. The action mechanism maybe related to the activation of PI3K/AKT signal pathways.
出处
《世界科学技术-中医药现代化》
2015年第6期1194-1197,共4页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
国家自然科学基金委面上项目(81273949/H2902):丹蒌片促进兔髂动脉粥样硬化药物涂层支架植入后再内皮化的调控机制研究
负责人:关怀敏
河南省科技厅基础与前言立项(132300410154):基于ERS/NF-κB信号通路的丹蒌片抗动脉粥样硬化机制研究
负责人:沈晓君
河南省教育厅高等学校重点科研项目(14A320051):经光学相干断层成像(OCT)观察丹蒌片促进PCI术后再内皮化的作用
负责人:陈玉善
郑州市科技局科技创新团队(121PCXTD520):中药防治郑州市重大疾病相关机制研究
负责人:朱艳琴
