摘要
目的：建立同时测定枇芩口服液中黄芩苷、黄芩素和汉黄芩素含量的高效液相色谱（ HPLC ）法。方法色谱柱为Kromasil－C18柱（250 mm ＆#215;4.6 mm，5μm），流动相为甲醇－0.2%磷酸溶液梯度洗脱，流速为1.0 mL／min，进样量为10μL，检测波长280 nm，柱温35℃。结果枇芩口服液中的黄芩苷、黄芩素、汉黄芩素达到了完全分离，黄芩苷、黄芩素、汉黄芩素的质量浓度线性范围分别为34.20~171.00μg／mL（ r=0.9998），1.44~7.20μg／mL（ r =0.9996），1.36~6.80μg／mL（ r=0.9977）；高、中、低浓度样品的平均回收率，黄芩苷分别为99.09%、99.56%和99.61%，黄芩素为98.46%、97.95%、98.31%，汉黄芩素为98.44%、98.51%和98.10%（ n=9）。结论该法操作简单、准确、重复性好，可用于枇芩口服液的质量控制。
Objective To establisb a metbod for tbe simultaneous determination of baicalin, baicalein and bogonin in Piqin Oral Solution by HPLC. Methods Tbe Kromasil-C18 column （ 250 mm ＆#215; 4. 6 mm, 5 μm ） witb a column temperature of 35 ℃ was used：tbe mobile pbase consisted of metbanol-0. 2% pbospbate, gradient elution, witb flow rate of 1. 0 mL/min, and tbe detection wavelengtb was set at 280 nm. Results Tbe content of baicalin, baicalein and wogonin acbieved a complete separation, peak area of tbe 3 components bad a good linear relationsbip witb tbe amount of samples. Tbe linear ranges of baicalin, baicalein and wogonin were 34. 20-171. 00 μg/mL （ r=0. 999 8）, 1. 44-7. 20 μg/mL（ r=0. 999 6）, 1. 36-6. 80 μg/mL（ r=0. 997 7） respectively;tbe sample-adding recovery were 99. 09%, 99. 56%, 99. 61% for baicalin,98. 46%, 97. 95%, 98. 31% for baicalein,and 98. 44, 98. 51, 98. 10% for wogonin in bigb,middle, low concentrations（ n=9）. Conclusion Tbe metbod is simple, rapid, accurate and reproducible, and it is suitable for tbe content determination of baicalin, baicalein and wogonin in Piqin Oral Solution.
出处
《中国药业》
CAS
2015年第16期93-95,共3页
China Pharmaceuticals
